Hetta Helal F, Mwafey Ibrahim M, Batiha Gaber El-Saber, Alomar Suliman Y, Mohamed Nahed A, Ibrahim Maggie A, Elkady Abeer, Meshaal Ahmed Kh, Alrefai Hani, Khodeer Dina M, Zahran Asmaa M
Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0595, USA.
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt.
Vaccines (Basel). 2020 Jun 26;8(2):340. doi: 10.3390/vaccines8020340.
Regulatory B cells (Bregs) are unique subpopulations of B cells with immune-regulating or immune-suppressing properties and play a role in peripheral tolerance. Due to the current limitations of human Breg studies among periodontal diseases, in the present study, we tried to analyze the change in circulating Bregs, pro-inflammatory, and anti-inflammatory cytokines in patients with periodontitis. Peripheral blood from 55 patients with stage 2 periodontitis and 20 healthy controls was analyzed using flow cytometry to evaluate the frequency of CD19CD24CD38 Breg cells. ELISA was used to assess the serum levels of the pro-inflammatory cytokines, including interleukins (IL)-1β, IL-6, TNF-α, and anti-inflammatory cytokines including IL-10, IL-35, and TGF-β. Increased proportions of Breg cells were observed in patients with stage 2 periodontitis compared to controls. Serum levels of cytokines were significantly higher in patients with periodontitis compared to controls. A significant positive correlation was observed between the frequencies of Breg cells and IL35 levels, IL10 levels, and TGF-β. In conclusion, our results suggest that the increase in peripheral Breg cells and serum cytokine levels among periodontitis patients seems to be closely associated with disease progression, a possible link between periodontitis, and systemic inflammatory process.
调节性B细胞（Bregs）是具有免疫调节或免疫抑制特性的独特B细胞亚群，在外周耐受中发挥作用。由于目前人类Breg细胞在牙周疾病研究方面存在局限性，在本研究中，我们试图分析牙周炎患者循环Bregs、促炎和抗炎细胞因子的变化。使用流式细胞术分析了55例2期牙周炎患者和20例健康对照者的外周血，以评估CD19CD24CD38 Breg细胞的频率。采用酶联免疫吸附测定（ELISA）法评估促炎细胞因子（包括白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α）和抗炎细胞因子（包括IL-10、IL-35和转化生长因子-β）的血清水平。与对照组相比，2期牙周炎患者中Breg细胞比例增加。与对照组相比，牙周炎患者的细胞因子血清水平显著更高。Breg细胞频率与IL35水平、IL10水平和转化生长因子-β之间存在显著正相关。总之，我们的结果表明，牙周炎患者外周Breg细胞和血清细胞因子水平的升高似乎与疾病进展密切相关，这可能是牙周炎与全身炎症过程之间的一个联系。
