载脂蛋白 E 基因敲除小鼠高脂血症模型中高迁移率族蛋白 B1 与血栓形成的关系。
Association Between HMGB1 and Thrombogenesis in a Hyperlipaemia-induced Microminipig Model of Atherosclerosis.
机构信息
Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
Department of Comparative Animal Science, College of Life Science, Kurashiki University of Science and The Arts, Okayama, Japan.
出版信息
In Vivo. 2020 Jul-Aug;34(4):1871-1874. doi: 10.21873/invivo.11982.
BACKGROUND/AIM: An appropriate animal model is essential to investigate the relationship between inflammation, atherosclerosis, and thrombogenesis, and the development of preventive measures and therapies for atherosclerosis.
MATERIALS AND METHODS
Atherosclerosis was induced in Microminipigs (MMPs) using a high-fat diet. We assessed high mobility group box 1 (HMGB1) expression levels and measured thrombus formation using a Total Thrombus Formation Analysis System (T-TAS). MMPs were divided into a normal diet (control) group and four high-fat diet groups, with differing amounts of cholesterol. After 8 weeks, blood was collected for analysis.
RESULTS
HMGB1 levels increased with increasing dietary cholesterol, and a negative correlation was found between HMGB1 levels and thrombus formation time.
CONCLUSION
T-TAS is useful in the assessment of thrombogenesis in MMPs and HMGB1 is associated with thrombus formation.
背景/目的：建立合适的动物模型对于研究炎症、动脉粥样硬化和血栓形成之间的关系，以及开发动脉粥样硬化的预防措施和治疗方法至关重要。
材料和方法
采用高脂饮食诱导小型猪（Microminipigs，MMPs）发生动脉粥样硬化。我们使用全血栓形成分析系统（Total Thrombus Formation Analysis System，T-TAS）评估高迁移率族蛋白 B1（high mobility group box 1，HMGB1）的表达水平并测量血栓形成。将 MMPs 分为正常饮食（对照组）组和四个不同胆固醇含量的高脂饮食组。8 周后采集血液进行分析。
结果
HMGB1 水平随饮食胆固醇的增加而升高，且 HMGB1 水平与血栓形成时间呈负相关。
结论
T-TAS 可用于评估 MMPs 中的血栓形成，且 HMGB1 与血栓形成有关。
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