Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan.
Fisheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Palembang, Ogan Ilir Regency, Indonesia.
Int J Nanomedicine. 2020 Jun 15;15:4191-4203. doi: 10.2147/IJN.S252885. eCollection 2020.
To characterize the nanoparticle of antroquinonol from and its ameliorative effects on the reproductive dysfunction in the diabetic male rat.
The chitosan-silicate nanoparticle was used as the carrier for the delivery of antroquinonol from solid-state-cultured extract (AC). The rats were fed with a high-fat diet and intraperitoneally injected with streptozotocin to induce diabetes. The rats were daily oral gavage by water [Diabetes (DM) and Control groups], three different doses of chitosan-silicate nanoparticle of antroquinonol from solid-state-cultured . (nano-SAC, NAC): (DM+NAC1x, 4 mg/kg of body weight; DM+NAC2x, 8 mg/kg; and DM+NAC5x, 20 mg/kg), solid-state-cultured AC (DM+AC5x, 20 mg/kg), or metformin (DM+Met, 200 mg/kg) for 7 weeks.
The nano-SAC size was 37.68±5.91 nm, the zeta potential was 4.13±0.49 mV, encapsulation efficiency was 79.29±0.77%, and loading capacity was 32.45±0.02%. The nano-SAC can improve diabetes-induced reproductive dysfunction by regulating glucose, insulin, and oxidative enzyme and by increasing the level of testosterone, follicle-stimulating hormone, luteinizing hormone, and sperm count as well as sperm mobility. In testicular histopathology, the seminiferous tubules of -supplemented diabetic rats showed similar morphology with the control group.
The nanoparticle of antroquinonol from can be used as an effective strategy to improve diabetes-induced testicular dysfunction.
从固态培养的 中提取的antroquinonol 纳米颗粒，并研究其对糖尿病雄性大鼠生殖功能障碍的改善作用。
壳聚糖-硅酸钠纳米颗粒被用作载体，用于递送来自固态培养 的 提取物（AC）中的 antroquinonol。大鼠喂食高脂肪饮食，并腹腔注射链脲佐菌素诱导糖尿病。大鼠每天用水进行口服灌胃[糖尿病（DM）和对照组]、三种不同剂量的固态培养 的壳聚糖-硅酸钠 antroquinonol 纳米颗粒（nano-SAC，NAC）：（DM+NAC1x，4mg/kg 体重；DM+NAC2x，8mg/kg；DM+NAC5x，20mg/kg）、固态培养的 AC（DM+AC5x，20mg/kg）或二甲双胍（DM+Met，200mg/kg），持续 7 周。
nano-SAC 的粒径为 37.68±5.91nm，zeta 电位为 4.13±0.49mV，包封效率为 79.29±0.77%，载药量为 32.45±0.02%。nano-SAC 可以通过调节葡萄糖、胰岛素和氧化酶，增加睾酮、卵泡刺激素、黄体生成素和精子计数以及精子活力，改善糖尿病引起的生殖功能障碍。在睾丸组织病理学检查中，补充 -的糖尿病大鼠的精小管形态与对照组相似。
来自固态培养的 antroquinonol 纳米颗粒可以作为一种有效的策略，改善糖尿病引起的睾丸功能障碍。
