Nanoparticles of Antroquinonol-Rich Extract from Solid-State-Cultured Improve Reproductive Function in Diabetic Male Rats.

PURPOSE

To characterize the nanoparticle of antroquinonol from and its ameliorative effects on the reproductive dysfunction in the diabetic male rat.

MATERIAL AND METHODS

The chitosan-silicate nanoparticle was used as the carrier for the delivery of antroquinonol from solid-state-cultured extract (AC). The rats were fed with a high-fat diet and intraperitoneally injected with streptozotocin to induce diabetes. The rats were daily oral gavage by water [Diabetes (DM) and Control groups], three different doses of chitosan-silicate nanoparticle of antroquinonol from solid-state-cultured . (nano-SAC, NAC): (DM+NAC1x, 4 mg/kg of body weight; DM+NAC2x, 8 mg/kg; and DM+NAC5x, 20 mg/kg), solid-state-cultured AC (DM+AC5x, 20 mg/kg), or metformin (DM+Met, 200 mg/kg) for 7 weeks.

RESULTS

The nano-SAC size was 37.68±5.91 nm, the zeta potential was 4.13±0.49 mV, encapsulation efficiency was 79.29±0.77%, and loading capacity was 32.45±0.02%. The nano-SAC can improve diabetes-induced reproductive dysfunction by regulating glucose, insulin, and oxidative enzyme and by increasing the level of testosterone, follicle-stimulating hormone, luteinizing hormone, and sperm count as well as sperm mobility. In testicular histopathology, the seminiferous tubules of -supplemented diabetic rats showed similar morphology with the control group.

CONCLUSION

The nanoparticle of antroquinonol from can be used as an effective strategy to improve diabetes-induced testicular dysfunction.