Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.
Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.
Biomed Pharmacother. 2019 Apr;112:108684. doi: 10.1016/j.biopha.2019.108684. Epub 2019 Feb 21.
Diabetes mellitus together with the oxidative stress affects the process of spermatogenesis and leads to male infertility. Antrodia cinnamomea (AC) is a mushroom found unique in Taiwan and commonly used for the treatment of several types of cancers and inflammatory disorders. This study was aimed to investigate the anti-oxidative and the ameliorative effects of Antrodia cinnamomea ethanol extract (ACEE) on reproduction dysfunction in male diabetic rats. The diabetic condition was induced by administrating the combination of streptozotocin (STZ) (65 mg/kg) and nicotinamide (NA) (230 mg/kg). Three different doses of ACEE were tested (385, 770, 1540 mg/kg) for 5 weeks. The results indicated that the ACEE improved STZ-NA induced hyperglycemia, oxidative stress, and insulin resistance. In addition to this, ACEE reduced the degree of lipid peroxidation, recovered the abnormal structure of the seminiferous tubules, and improved sperm parameters. Moreover, the DNA damages and mitochondrial membrane potential were improved in sperm. Our study confirmed that the ACEE has anti-inflammatory and ameliorative effects to prevent diabetes-induced male reproductive dysfunction.
糖尿病和氧化应激会影响精子发生过程，导致男性不育。樟芝（Antrodia cinnamomea，AC）是台湾特有的一种蘑菇，常用于治疗多种癌症和炎症性疾病。本研究旨在探讨樟芝乙醇提取物（Antrodia cinnamomea ethanol extract，ACEE）对糖尿病雄性大鼠生殖功能障碍的抗氧化和改善作用。糖尿病状态通过给予链脲佐菌素（streptozotocin，STZ）（65mg/kg）和烟酰胺（nicotinamide，NA）（230mg/kg）联合诱导。测试了 ACEE 的三种不同剂量（385、770、1540mg/kg），为期 5 周。结果表明，ACEE 改善了 STZ-NA 诱导的高血糖、氧化应激和胰岛素抵抗。除此之外，ACEE 还降低了脂质过氧化程度，恢复了曲细精管的异常结构，并改善了精子参数。此外，精子的 DNA 损伤和线粒体膜电位也得到了改善。我们的研究证实，ACEE 具有抗炎和改善作用，可以预防糖尿病引起的男性生殖功能障碍。
