Mao Chien-Feng, Sudirman Sabri, Lee Chi-Chih, Tsou David, Kong Zwe-Ling
Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.
Fisheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Palembang, Indonesia.
Front Vet Sci. 2021 Apr 28;8:651286. doi: 10.3389/fvets.2021.651286. eCollection 2021.
As lifestyle changes, the prevalence of diabetes increases every year. Diabetes-induced male reproductive dysfunction is predominantly due to increased oxidative stress and then results in sperm damage and infertility. is a traditional medicinal herb and is well-known for its immune-modulatory, antioxidative, anti-inflammatory, anticancer, and antiviral activities. The Toll-like receptor 4 (TLR4) plays a critical role in innate immune responses leading to nuclear factor (NF)-κB phosphorylation and release of proinflammatory cytokines including nitric oxide (NO), interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. However, the relation between extract and TLR4 remains unclear. This study aimed to investigate the protective effects on male reproduction of ethanol extract (EPE) against diabetic rats and whether the anti-inflammatory effects were through the TLR4 pathway. Diabetic male Sprague-Dawley (SD) rats were induced by streptozotocin (65 mg/kg) and nicotinamide (230 mg/kg). EPE was tested in three doses (93, 279, and 465 mg/kg p.o. daily) for 4 weeks. Besides, metformin administration (100 mg/kg/day) was treated as a positive control. Results indicated that EPE administration for about 4 weeks improved hyperglycemia and insulin resistance. Additionally, EPE increased sperm motility, protected sperm morphology and mitochondrial membrane potential, as well as protein for testosterone synthesis enzyme. In sperm superoxide dismutase, catalase, and glutathione antioxidants were increased, whereas proinflammatory cytokines, such as NO, IL-1β, and TNF-α were decreased. The testis protein content of TLR4 and downstream phospho-NF-κB p65 also were reduced. The EPE might reduce the production of proinflammatory cytokines TLR4 pathways and improve diabetes-induced male infertility.
随着生活方式的改变，糖尿病的患病率逐年上升。糖尿病引起的男性生殖功能障碍主要是由于氧化应激增加，进而导致精子损伤和不育。[某种草药名称未给出]是一种传统草药，以其免疫调节、抗氧化、抗炎、抗癌和抗病毒活性而闻名。Toll样受体4（TLR4）在导致核因子（NF）-κB磷酸化和促炎细胞因子释放（包括一氧化氮（NO）、白细胞介素（IL）-1β和肿瘤坏死因子（TNF）-α）的先天免疫反应中起关键作用。然而，[草药名称未给出]提取物与TLR4之间的关系仍不清楚。本研究旨在探讨[草药名称未给出]乙醇提取物（EPE）对糖尿病大鼠雄性生殖的保护作用，以及其抗炎作用是否通过TLR4途径。雄性Sprague-Dawley（SD）糖尿病大鼠由链脲佐菌素（65 mg/kg）和烟酰胺（230 mg/kg）诱导产生。EPE以三种剂量（每天口服93、279和465 mg/kg）进行测试，持续4周。此外，给予二甲双胍（100 mg/kg/天）作为阳性对照。结果表明，给予EPE约4周可改善高血糖和胰岛素抵抗。此外，EPE增加了精子活力，保护了精子形态和线粒体膜电位，以及睾酮合成酶的蛋白质。精子中超氧化物歧化酶、过氧化氢酶和谷胱甘肽抗氧化剂增加，而促炎细胞因子如NO、IL-1β和TNF-α减少。睾丸中TLR4和下游磷酸化NF-κB p65的蛋白质含量也降低。EPE可能通过TLR4途径减少促炎细胞因子的产生，改善糖尿病引起的男性不育。
