Simões E Silva Lucas Lucena, Santos de Sousa Fernandes Matheus, Kubrusly Márcia Saldanha, Muller Cynthia Rodrigues, Américo Anna Laura Viacava, Stefano Jose Tadeu, Evangelista Fabiana Sant'Anna, Oliveira Claudia Pinto, Jukemura José
Division of Gastroenterology and Hepatology, Department of Gastroenterology (LIM 07), University of São Paulo School of Medicine, São Paulo, Brazil.
Department of Experimental Pathophysiology, Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
Clin Exp Gastroenterol. 2020 Jun 19;13:223-234. doi: 10.2147/CEG.S242393. eCollection 2020.
To evaluate the effect of 8 weeks of aerobic training on insulin resistance and inflammatory response in obese mice (ob/ob) with NAFLD.
Male ob/ob mice were randomly divided into sedentary (n=7) and trained (n=7) groups. Aerobic training consisted of 5 weekly sessions, 60 min per session at 60% of the maximum speed of the running test. Hepatic and pancreatic samples were collected to evaluate histological features and gene expression associated with insulin resistance and inflammatory response after 8-week experiment protocol. RNA was performed by TRIzol. PCR experiments were performed using the Rotor-Gene RG-3000. Parametric data were assessed by -test, one-way ANOVA and Bonferroni test for multiple comparisons. Non-parametric data were assessed by the Mann-Whitney tests with Dunn's post-test of multiple comparisons. Histological analysis was assessed by chi-square test with Fisher's exact test. Significant variables were considered when p<0.05. All the analyses were performed by GraphPad Prism V6.0 software (GraphPad Software Inc.).
Reductions in bodyweight (p = 0.008), weight evolution (p = 0.03), food intake (p <0.0001) and fat content were observed in trained group. Moreover, the trained group showed better results in peak velocity (=0.03) physical effort tolerance (p=0.006) and distance (p=0.01). Gene expression showed differences in IL-10 (p=0.03) and GLUT-2 (p=0.03) in hepatic analysis, between groups. Pancreatic gene expression showed difference between groups in IRS-2 (p=0.004), GLUT-2 (p=0.03) and IL-10 (p=0.008) analysis. Also, the trained group showed lower values for interlobular fat and inflammatory infiltrate in histological analysis when compared to sedentary animals.
An 8-week physical training protocol was able to attenuate bodyweight gain, food intake and generate positive effects on gene expression related to insulin resistance and inflammation in both liver and pancreas of ob/ob mice.
评估为期8周的有氧运动训练对患有非酒精性脂肪性肝病（NAFLD）的肥胖小鼠（ob/ob）胰岛素抵抗和炎症反应的影响。
将雄性ob/ob小鼠随机分为久坐组（n = 7）和训练组（n = 7）。有氧运动训练包括每周5次训练，每次训练60分钟，速度为跑步测试最大速度的60%。在为期8周的实验方案结束后，收集肝脏和胰腺样本，以评估与胰岛素抵抗和炎症反应相关的组织学特征和基因表达。使用TRIzol提取RNA。使用Rotor-Gene RG-3000进行PCR实验。参数数据通过t检验、单因素方差分析和Bonferroni检验进行多重比较评估。非参数数据通过Mann-Whitney检验和Dunn's事后多重比较检验进行评估。组织学分析通过卡方检验和Fisher精确检验进行评估。当p<0.05时，认为变量具有显著性。所有分析均使用GraphPad Prism V6.0软件（GraphPad Software Inc.）进行。
训练组的体重（p = 0.008）、体重变化（p = 0.03）、食物摄入量（p <0.0001）和脂肪含量均有所降低。此外，训练组在峰值速度（p = 0.03）、体力耐力（p = 0.006）和距离（p = 0.01）方面表现更好。基因表达显示，两组之间肝脏分析中的IL-10（p = 0.03）和GLUT-2（p = 0.03）存在差异。胰腺基因表达显示，两组之间在IRS-2（p = 0.004）、GLUT-2（p = 0.03）和IL-10（p = 0.008）分析中存在差异。此外，与久坐的动物相比，训练组在组织学分析中的小叶间脂肪和炎症浸润值更低。
为期8周的体育训练方案能够减轻ob/ob小鼠的体重增加和食物摄入量，并对肝脏和胰腺中与胰岛素抵抗和炎症相关的基因表达产生积极影响。
