Research Institute of Water and Environmental Engineering (IIAMA), Universitat Politècnica de València, Camino de Vera 14, 46022, Valencia, Spain.
Microbiology Department, Science Faculty, Pontificia Universidad Javeriana, Bogotá, Colombia.
Int Microbiol. 2020 Nov;23(4):481-487. doi: 10.1007/s10123-020-00135-z. Epub 2020 Jun 30.
The objective of the present study was to establish the most suitable culture medium for the isolation of H. pylori from environmental and clinical samples. Ten different culture media were compared and evaluated. Four of them had been previously described and were modified in this study. The rest of the media were designed de novo. Three different matrices, tap water, wastewater, and feces, were inoculated with serial dilutions of H. pylori NCTC 11637 strain at a final concentration of 10 and 10 CFU/ml and the recovery rates were calculated. From inoculated tap water and wastewater samples, H. pylori colonies were recovered from four out of the analyzed culture media. When fecal samples were analyzed, the isolation of the pathogen under study was only possible from two culture media. Different optimal media were observed for each type of sample, even for wastewater and stool samples. Nevertheless, our results indicated that the combination of Dent Agar with polymyxin B sulfate did not inhibit the growth of H. pylori and was highly selective for its recovery, regardless of the sample origin. Thus, we propose the use of this medium as a diagnostic tool for the isolation of H. pylori from environmental and clinical samples, as well as for epidemiological studies.
本研究的目的是建立最适合从环境和临床样本中分离幽门螺杆菌的培养基。比较和评估了十种不同的培养基。其中四种是以前描述过的，并在本研究中进行了修改。其余的培养基是全新设计的。将三种不同的基质（自来水、废水和粪便）用幽门螺杆菌 NCTC 11637 菌株的连续稀释液接种，终浓度为 10 和 10 CFU/ml，并计算回收率。从接种的自来水和废水样本中，从分析的四种培养基中回收了幽门螺杆菌的菌落。分析粪便样本时，仅能从两种培养基中分离出研究中的病原体。即使对于废水和粪便样本，也观察到每种类型的样本都有不同的最佳培养基。然而，我们的结果表明，多粘菌素 B 硫酸盐与 Dent 琼脂的组合不会抑制幽门螺杆菌的生长，并且对其回收具有高度选择性，而与样本来源无关。因此，我们建议将该培养基用作从环境和临床样本中分离幽门螺杆菌以及进行流行病学研究的诊断工具。
