使用粪便聚合酶链反应检测患者幽门螺杆菌感染。
Use of PCR with feces for detection of Helicobacter pylori infections in patients.
作者信息
van Zwet A A, Thijs J C, Kooistra-Smid A M, Schirm J, Snijder J A
机构信息
Regional Public Health Laboratory, Groningen, The Netherlands.
出版信息
J Clin Microbiol. 1994 May;32(5):1346-8. doi: 10.1128/jcm.32.5.1346-1348.1994.
Abstract
PCR was performed for the detection of Helicobacter pylori in feces from 24 patients with proven infections. Several precautions were taken to overcome possible inhibition of PCR with feces. In the first 12 patients, feces were examined shortly after endoscopy. In another group of 12 patients, who were treated during 2 weeks with omeprazole (40 mg each day) to increase gastric pH, feces were examined as well. H. pylori target DNA could not be detected in the stools of any of the 24 infected patients. It was concluded that there was no substantial shedding of H. pylori in feces from either group of patients.
摘要
对24例已确诊感染的患者粪便进行聚合酶链反应（PCR）检测幽门螺杆菌。采取了多项预防措施以克服粪便对PCR可能产生的抑制作用。前12例患者在内镜检查后不久即检查粪便。另一组12例患者，在两周内每天服用奥美拉唑（40毫克）以提高胃内pH值，同时也检查了粪便。24例感染患者的粪便中均未检测到幽门螺杆菌目标DNA。得出的结论是，两组患者的粪便中均未大量排出幽门螺杆菌。
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