使用粪便聚合酶链反应检测患者幽门螺杆菌感染。
Use of PCR with feces for detection of Helicobacter pylori infections in patients.
作者信息
van Zwet A A, Thijs J C, Kooistra-Smid A M, Schirm J, Snijder J A
机构信息
Regional Public Health Laboratory, Groningen, The Netherlands.
出版信息
J Clin Microbiol. 1994 May;32(5):1346-8. doi: 10.1128/jcm.32.5.1346-1348.1994.
Abstract
PCR was performed for the detection of Helicobacter pylori in feces from 24 patients with proven infections. Several precautions were taken to overcome possible inhibition of PCR with feces. In the first 12 patients, feces were examined shortly after endoscopy. In another group of 12 patients, who were treated during 2 weeks with omeprazole (40 mg each day) to increase gastric pH, feces were examined as well. H. pylori target DNA could not be detected in the stools of any of the 24 infected patients. It was concluded that there was no substantial shedding of H. pylori in feces from either group of patients.
摘要
对24例已确诊感染的患者粪便进行聚合酶链反应（PCR）检测幽门螺杆菌。采取了多项预防措施以克服粪便对PCR可能产生的抑制作用。前12例患者在内镜检查后不久即检查粪便。另一组12例患者，在两周内每天服用奥美拉唑（40毫克）以提高胃内pH值，同时也检查了粪便。24例感染患者的粪便中均未检测到幽门螺杆菌目标DNA。得出的结论是，两组患者的粪便中均未大量排出幽门螺杆菌。
相似文献
1
Use of PCR with feces for detection of Helicobacter pylori infections in patients.使用粪便聚合酶链反应检测患者幽门螺杆菌感染。
J Clin Microbiol. 1994 May;32(5):1346-8. doi: 10.1128/jcm.32.5.1346-1348.1994.
2
Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR.纤维内镜清洗程序对通过聚合酶链反应检测胃活检标本中幽门螺杆菌的重要性。
J Clin Microbiol. 1994 Apr;32(4):1123-6. doi: 10.1128/jcm.32.4.1123-1126.1994.
3
Detection of Helicobacter pylori DNA in gastric juice by the polymerase chain reaction: comparison with findings in bacterial culture and the detection of tissue IgA and serum IgG antibodies against Helicobacter pylori.通过聚合酶链反应检测胃液中的幽门螺杆菌DNA：与细菌培养结果以及针对幽门螺杆菌的组织IgA和血清IgG抗体检测结果的比较。
J Gastroenterol. 1995 Dec;30(6):689-95. doi: 10.1007/BF02349633.
4
Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori.使用引物Hp1和Hp2的16S rRNA基因PCR检测幽门螺杆菌的评估。
J Clin Microbiol. 1996 Nov;34(11):2728-30. doi: 10.1128/jcm.34.11.2728-2730.1996.
5
PCR detection of colonization by Helicobacter pylori in conventional, euthymic mice based on the 16S ribosomal gene sequence.基于16S核糖体基因序列，通过聚合酶链反应检测常规健康小鼠中幽门螺杆菌的定植情况。
Clin Diagn Lab Immunol. 1996 Jan;3(1):66-72. doi: 10.1128/cdli.3.1.66-72.1996.
6
[Comparison of four different primer sets for detection of Helicobacter pylori in gastric biopsies and oral samples by using real-time PCR].[使用实时聚合酶链反应比较四种不同引物组检测胃活检组织和口腔样本中幽门螺杆菌的情况]
Pathol Biol (Paris). 2009 Feb;57(1):30-5. doi: 10.1016/j.patbio.2008.07.008. Epub 2008 Oct 7.
7
Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies.从胃内镜活检组织中直接检测和扩增幽门螺杆菌核糖体16S基因片段。
Diagn Microbiol Infect Dis. 1990 Nov-Dec;13(6):473-9. doi: 10.1016/0732-8893(90)90079-b.
8
Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori.使用引物Hp1和Hp2的16S rRNA基因聚合酶链反应检测幽门螺杆菌的评估。
J Clin Microbiol. 1998 Feb;36(2):603. doi: 10.1128/JCM.36.2.603-603.1998.
9
Evaluation of a PCR primer based on the isocitrate dehydrogenase gene for detection of Helicobacter pylori in feces.基于异柠檬酸脱氢酶基因的PCR引物用于粪便中幽门螺杆菌检测的评估
J Clin Microbiol. 2000 Oct;38(10):3755-8. doi: 10.1128/JCM.38.10.3755-3758.2000.
10
Application of stool-PCR test for diagnosis of Helicobacter pylori infection in children.粪便聚合酶链反应检测在儿童幽门螺杆菌感染诊断中的应用。
World J Gastroenterol. 2009 Jan 28;15(4):484-8. doi: 10.3748/wjg.15.484.
引用本文的文献
1
Neonatal infection with affects stomach and colon microbiome composition and gene expression in mice.新生儿感染 影响小鼠的胃和结肠微生物群组成及基因表达。 （注：原文中“with”后面缺少具体感染因素，翻译可能会有信息不完整之感）
bioRxiv. 2025 May 5:2025.04.30.650706. doi: 10.1101/2025.04.30.650706.
2
Helicobacter pylori infection associates with fecal microbiota composition and diversity.幽门螺杆菌感染与粪便微生物群落组成和多样性相关。
Sci Rep. 2019 Dec 27;9(1):20100. doi: 10.1038/s41598-019-56631-4.
3
Non-invasive diagnostic tests for Helicobacter pylori infection.幽门螺杆菌感染的非侵入性诊断测试。
Cochrane Database Syst Rev. 2018 Mar 15;3(3):CD012080. doi: 10.1002/14651858.CD012080.pub2.
4
Ulcerative Colitis and Its Association with Salmonella Species.溃疡性结肠炎及其与沙门氏菌属的关联。
Interdiscip Perspect Infect Dis. 2016;2016:5854285. doi: 10.1155/2016/5854285. Epub 2016 Jan 20.
5
Mix-infection of S. Typhi and ParaTyphi A in Typhoid Fever and Chronic Typhoid Carriers: A Nested PCR Based Study in North India.伤寒热和慢性伤寒带菌者中伤寒沙门氏菌和甲型副伤寒沙门氏菌的混合感染：印度北部一项基于巢式PCR的研究
J Clin Diagn Res. 2014 Nov;8(11):DC09-14. doi: 10.7860/JCDR/2014/9167.5107. Epub 2014 Nov 20.
6
Acid exposure induces multiplication of Salmonella enterica serovar Typhi.酸暴露诱导伤寒沙门氏菌血清型 Typhi 的增殖。
J Clin Microbiol. 2014 Dec;52(12):4330-3. doi: 10.1128/JCM.02275-14. Epub 2014 Oct 15.
7
Detection of Helicobacter pylori using nested polymerase chain reaction in gastric biopsy samples.在胃活检样本中使用巢式聚合酶链反应检测幽门螺杆菌。
Indian J Clin Biochem. 2008 Jul;23(3):243-5. doi: 10.1007/s12291-008-0054-y. Epub 2008 Oct 1.
8
Use of urine with nested PCR targeting the flagellin gene (fliC) for diagnosis of typhoid fever.应用Nested-PCR 技术检测鞭毛蛋白基因（fliC）在尿标本中诊断伤寒。
J Clin Microbiol. 2012 Jun;50(6):1964-7. doi: 10.1128/JCM.00031-12. Epub 2012 Apr 4.
9
Helicobacter pylori: a poor man's gut pathogen?幽门螺杆菌：穷人的肠道病原体？
Gut Pathog. 2010 Mar 31;2(1):2. doi: 10.1186/1757-4749-2-2.
10
Helicobacter pylori detection and antimicrobial susceptibility testing.幽门螺杆菌检测及抗菌药物敏感性试验。
Clin Microbiol Rev. 2007 Apr;20(2):280-322. doi: 10.1128/CMR.00033-06.
本文引用的文献
1
Role of gastric pH in isolation of Helicobacter mustelae from the feces of ferrets.胃内pH值在从雪貂粪便中分离幽门螺杆菌中的作用。
Gastroenterology. 1993 Jan;104(1):86-92. doi: 10.1016/0016-5085(93)90839-5.
2
Sensitivity of culture compared with that of polymerase chain reaction for detection of Helicobacter pylori from antral biopsy samples.与聚合酶链反应相比，从胃窦活检样本中检测幽门螺杆菌的培养敏感性。
J Clin Microbiol. 1993 Jul;31(7):1918-20. doi: 10.1128/jcm.31.7.1918-1920.1993.
3
PCR identification of Helicobacter pylori in faeces from gastritis patients.胃炎患者粪便中幽门螺杆菌的聚合酶链反应鉴定
Lancet. 1993 Feb 13;341(8842):447. doi: 10.1016/0140-6736(93)93053-4.
4
Transmission of Helicobacter pylori via faeces.
Lancet. 1993 Dec 4;342(8884):1419-20. doi: 10.1016/0140-6736(93)92775-o.
5
Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH.摄入幽门弯曲杆菌会导致胃炎并使空腹时胃的pH值升高。
Am J Gastroenterol. 1987 Mar;82(3):192-9.
6
Iatrogenic Campylobacter pylori infection is a cause of epidemic achlorhydria.医源性幽门螺杆菌感染是流行性胃酸缺乏症的一个病因。
Am J Gastroenterol. 1988 Sep;83(9):974-80.
7
Seroprevalence of Helicobacter pylori infections in Thailand.泰国幽门螺杆菌感染的血清流行率。
J Infect Dis. 1990 Jun;161(6):1237-41. doi: 10.1093/infdis/161.6.1237.
8
Epidemiology of Helicobacter pylori in an asymptomatic population in the United States. Effect of age, race, and socioeconomic status.美国无症状人群中幽门螺杆菌的流行病学。年龄、种族和社会经济地位的影响。
Gastroenterology. 1991 Jun;100(6):1495-501. doi: 10.1016/0016-5085(91)90644-z.
9
Transmission of Helicobacter spp. A challenge to the dogma of faecal-oral spread.
Epidemiol Infect. 1991 Aug;107(1):99-109. doi: 10.1017/s095026880004872x.
10
Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals.用于检测人和动物幽门螺杆菌的直接聚合酶链反应试验
J Clin Microbiol. 1991 Nov;29(11):2543-9. doi: 10.1128/jcm.29.11.2543-2549.1991.
Zotero 插件