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EI09 和硒共施缓解铬胁迫对 L. 的影响

Ameliorative effect of co-application of EI09 and Se to mitigate chromium stress in L.

机构信息

Department of Botany, University of the Narowal, Narowal, Pakistan.

Guangdong Key Laboratory for New Technology Research of Vegetables/Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

出版信息

Int J Phytoremediation. 2020;22(13):1396-1407. doi: 10.1080/15226514.2020.1780412. Epub 2020 Jul 1.

Abstract

The present study was conducted to explore the potential of EI09 (EI09) and selenium (Se) alone or in combination to mitigate hexavalent chromium (Cr) stress in L. Chromium stressed plants exhibited significant reduction in biomass, chlorophyll content and gas exchange characteristics. The inoculated seedlings subjected to Crstress showed improvement in growth, proline content, gas exchange attributes and total soluble proteins. Likewise, inoculated seedlings exhibited augmented activity of ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) under Cr stress. The Cr stress mitigation in inoculated seedlings was ascribed to reduction in malondialdehyde (MDA) content, hydrogen peroxide (HO) besides increase activity of flavonoids, proline, phenolic content along with modulation of antioxidative enzymes. The growth-enhancing attributes of bacteria such as indole acetic acid (IAA) content and 1-aminocyclopropane-1-carboxylate deaminase (ACCD) activity enhanced growth in Cr-stressed plants. Moreover, co-treatment of EI09 and 5 µM Se effectively mitigated Cr (VI) stress in plants. Current studies provide a novel insight into potential of EI09 and Se in reduction of Cr toxicity in plants.

摘要

本研究旨在探索 EI09 和硒(Se)单独或联合使用缓解六价铬(Cr)胁迫的潜力。在受到 Cr 胁迫的植物中,生物量、叶绿素含量和气体交换特性显著降低。接种的幼苗在受到 Cr 胁迫时,表现出生长、脯氨酸含量、气体交换特性和总可溶性蛋白的改善。同样,接种的幼苗在 Cr 胁迫下表现出增强的抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性。接种幼苗减轻 Cr 胁迫归因于丙二醛(MDA)含量和氢过氧化物(HO)的减少,以及类黄酮、脯氨酸、酚类含量的增加以及抗氧化酶的调节。细菌如吲哚乙酸(IAA)含量和 1-氨基环丙烷-1-羧酸脱氨酶(ACCD)活性等生长促进特性增强了 Cr 胁迫下植物的生长。此外,EI09 和 5 μM Se 的联合处理有效地减轻了植物中的 Cr(VI)胁迫。目前的研究为 EI09 和 Se 降低植物 Cr 毒性的潜力提供了新的见解。

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