Liu Duo, Wang Lei, Cao Zhan-Bo, Duan Hao
Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
Huan Jing Ke Xue. 2020 Feb 8;41(2):932-940. doi: 10.13227/j.hjkx.201906119.
To investigate the effect of mycorrhizal fungi inoculation on nitrification-denitrification in the rhizospheric soil of aquatic plants, and were inoculated with the strain MF-MD obtained from local soil. Uninoculated plants served as the control group. The plants were cultivated for 3 months under nitrogen eutrophication in water, and then the nitrification and denitrification activities in the rhizospheric soil were determined. The results showed that inoculation with MF-MD promoted nitrification in the rhizospheric soil of both plants. However, MF-MD inoculation promoted denitrification in the rhizospheric soil of but inhibited denitrification in the rhizospheric soil of . The mechanism of soil nitrification-denitrification activity was analyzed by measuring the changes in the community structure of nitrifying bacteria and denitrifying bacteria in the microbial biomass of the rhizospheric soil. It was found that the rhizospheric soil microbial biomass (SMB) of the experimental group was higher than that of the control group and that the changes in the microbial community structure related to soil nitrification and denitrification differed between groups. This study contributes to an understanding of how mycorrhizal fungi combined with aquatic plants can remove N content in eutrophic water.
为研究接种菌根真菌对水生植物根际土壤硝化-反硝化作用的影响，对从当地土壤中获得的菌株MF-MD进行接种。未接种的植物作为对照组。将植物在富氮水体中培养3个月，然后测定根际土壤中的硝化和反硝化活性。结果表明，接种MF-MD促进了两种植物根际土壤的硝化作用。然而，接种MF-MD促进了一种植物根际土壤的反硝化作用，但抑制了另一种植物根际土壤的反硝化作用。通过测量根际土壤微生物生物量中硝化细菌和反硝化细菌群落结构的变化，分析了土壤硝化-反硝化活性的机制。研究发现，实验组的根际土壤微生物生物量（SMB）高于对照组，且与土壤硝化和反硝化相关的微生物群落结构变化在不同组间存在差异。本研究有助于理解菌根真菌与水生植物如何联合去除富营养化水体中的氮含量。
