Department of Regenerative Medicine, University Institute for Regenerative Medicine and Oral Implantology (UIRMI), Vitoria, Spain,
Regenerative Medicine Laboratory, BTI - Biotechnology Institute, Vitoria, Spain,
Skin Pharmacol Physiol. 2020;33(3):67-81. doi: 10.1159/000507716. Epub 2020 Jul 1.
Skin as the major barrier between the internal and external environments protects our body from external injuries. Ultraviolet B (UVB) radiation is majorly responsible for photoaging and is closely associated with oxidative stress, inflammation, and DNA damage. The progression in the field of biological therapies has led to the emergence of new autologous therapies based on growth factors. An autologous topical serum (ATS) based on the plasma rich in growth factors (PRGF) technology has also been developed with regenerative properties.
The aim of this study was to evaluate this new topical formulation in protecting skin against UVB-induced photodamage using dermal fibroblast cultures and 3D skin models.
ATS was assessed over the main mechanisms underlying photodamage including oxidative stress, cell viability, DNA damage, cell death, and biosynthetic activity. Three different irradiation protocols were tested.
ATS application showed to significantly reduce free radical production and cell death caused by ultraviolet radiation. It also increased cell viability and promoted the proliferative activity and fibronectin biosynthesis of dermal fibroblasts. DNA double-strand cleavage that occurs after photo-oxidative stress was reduced. Photoexposed 3D explants showed higher levels of metabolic activity and collagen synthesis. Histomorphometric analysis also revealed a reduction in UV-derived edema, hyperkeratosis, and apoptosis and an increase in collagen and cell bioactivity.
This preliminary study suggests that this novel ATS might counteract the harmful effects of UV radiation.
皮肤作为人体内部与外部环境之间的主要屏障，保护我们的身体免受外部伤害。紫外线 B（UVB）辐射是导致光老化的主要原因，与氧化应激、炎症和 DNA 损伤密切相关。生物治疗领域的进展导致了基于生长因子的新型自体疗法的出现。一种基于富含生长因子的血浆（PRGF）技术的自体局部血清（ATS）也具有再生特性。
本研究旨在使用真皮成纤维细胞培养物和 3D 皮肤模型评估这种新的局部制剂对 UVB 诱导的光损伤的保护作用。
ATS 针对光损伤的主要机制进行了评估，包括氧化应激、细胞活力、DNA 损伤、细胞死亡和生物合成活性。测试了三种不同的辐照方案。
ATS 的应用显著减少了紫外线辐射引起的自由基产生和细胞死亡。它还提高了细胞活力，促进了真皮成纤维细胞的增殖活性和纤维连接蛋白的生物合成。减少了光氧化应激后发生的 DNA 双链断裂。经光暴露的 3D 外植体显示出更高的代谢活性和胶原合成水平。组织形态计量学分析还显示，UV 衍生的水肿、过度角化、细胞凋亡减少，胶原和细胞生物活性增加。
这项初步研究表明，这种新型 ATS 可能对抗紫外线辐射的有害影响。
