Anitua Eduardo, Pino Ander, Orive Gorka
Foundation Eduardo Anitua, Vitoria, Jacinto Quincoces, 39, 01007 Vitoria (Álava). Spain.
Curr Pharm Biotechnol. 2016;17(12):1068-1078. doi: 10.2174/1389201017666160709200920.
Ultraviolet irradiation is able to deeply penetrate into the dermis and alter fibroblast structure and function, leading to a degradation of the dermal extracellular matrix.
The regenerative effect of plasma rich in growth factors (PRGF) on skin ageing was investigated using UVB photo-stressed human dermal fibroblasts as an in vitro culture model.
PRGF was assessed over the main indicative features of ultraviolet B irradiation, including ROS formation, cell viability and death detection, apoptosis/ necrosis analysis and biosynthetic activity measurement. Four different UV irradiation protocols were tested in order to analyze the beneficial effects of PRGF.
Ultraviolet irradiation exhibited a dose dependent cytotoxicity and dose of 400mJ/cm2 was selected for subsequent experiments. PRGF increased the cell viability and decreased the cell death comparing to the non-treated group. The apoptosis and necrosis were significantly lower in PRGF treated fibroblasts. ROS production after UV irradiation was significantly reduced in the presence of PRGF. Procollagen type I, hyaluronic acid and TIMP-1 levels were higher in the when treated with PRGF.
This preliminary in vitro study suggests that PRGF is able to prevent UVB derived photooxidative stress and to diminish the cell damage caused by ultraviolet irradiation.
紫外线照射能够深入真皮层，改变成纤维细胞的结构和功能，导致真皮细胞外基质降解。
以紫外线B（UVB）光应激的人真皮成纤维细胞作为体外培养模型，研究富含生长因子的血浆（PRGF）对皮肤衰老的再生作用。
对PRGF进行紫外线B照射的主要指示特征评估，包括活性氧（ROS）形成、细胞活力和死亡检测、凋亡/坏死分析以及生物合成活性测量。测试了四种不同的紫外线照射方案，以分析PRGF的有益效果。
紫外线照射呈现剂量依赖性细胞毒性，后续实验选择400mJ/cm2的剂量。与未处理组相比，PRGF提高了细胞活力并降低了细胞死亡。PRGF处理的成纤维细胞中凋亡和坏死显著更低。在PRGF存在的情况下，紫外线照射后的ROS产生显著减少。用PRGF处理时，I型前胶原、透明质酸和金属蛋白酶组织抑制剂-1（TIMP-1）水平更高。
这项初步的体外研究表明，PRGF能够预防UVB引起的光氧化应激，并减少紫外线照射造成的细胞损伤。
