Xing Zhe, Cai Jiazheng, Sun Yang, Cao Mengnan, Li Yi, Xue Ying, Finne-Wistrand Anna, Kamal Mustafa
School of Stomatology, Lanzhou University, Lanzhou 730000, China.
Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, 5009 Bergen, Norway.
Polymers (Basel). 2020 Jun 29;12(7):1453. doi: 10.3390/polym12071453.
Recent studies have suggested that both poly(l-lactide--1,5-dioxepan-2-one) (or poly(LLA--DXO)) and poly(l-lactide--ε-caprolactone) (or poly(LLA--CL)) porous scaffolds are good candidates for use as biodegradable scaffold materials in the field of tissue engineering; meanwhile, their surface properties, such as hydrophilicity, need to be further improved.
We applied several different concentrations of the surfactant Tween 80 to tune the hydrophilicity of both materials. Moreover, the modification was applied not only in the form of solid scaffold as a film but also a porous scaffold. To investigate the potential application for tissue engineering, human bone marrow mesenchymal stem cells (hMSCs) were chosen to test the effect of hydrophilicity on cell attachment, proliferation, and differentiation. First, the cellular cytotoxicity of the extracted medium from modified scaffolds was investigated on HaCaT cells. Then, hMSCs were seeded on the scaffolds or films to evaluate cell attachment, proliferation, and osteogenic differentiation. The results indicated a significant increasing of wettability with the addition of Tween 80, and the hMSCs showed delayed attachment and spreading. PCR results indicated that the differentiation of hMSCs was stimulated, and several osteogenesis related genes were up-regulated in the 3% Tween 80 group. Poly(LLA--CL) with 3% Tween 80 showed an increased messenger Ribonucleic acid (mRNA) level of late-stage markers such as osteocalcin (OC) and key transcription factor as runt related gene 2 (Runx2).
A high hydrophilic scaffold may speed up the osteogenic differentiation for bone tissue engineering.
最近的研究表明，聚（L-丙交酯-1,5-二氧杂环庚烷-2-酮）（或聚（LLA-DXO））和聚（L-丙交酯-ε-己内酯）（或聚（LLA-CL））多孔支架是组织工程领域中用作可生物降解支架材料的良好候选者；同时，它们的表面性质，如亲水性，需要进一步改善。
我们应用几种不同浓度的表面活性剂吐温80来调节这两种材料的亲水性。此外，不仅以固体支架薄膜的形式进行改性，还对多孔支架进行改性。为了研究其在组织工程中的潜在应用，选择人骨髓间充质干细胞（hMSCs）来测试亲水性对细胞附着、增殖和分化的影响。首先，研究了从改性支架中提取的培养基对HaCaT细胞的细胞毒性。然后，将hMSCs接种在支架或薄膜上以评估细胞附着、增殖和成骨分化。结果表明，添加吐温80后润湿性显著增加，hMSCs表现出延迟的附着和铺展。PCR结果表明hMSCs的分化受到刺激，在3%吐温80组中几个与成骨相关的基因上调。含3%吐温80的聚（LLA-CL）显示晚期标志物如骨钙素（OC）的信使核糖核酸（mRNA）水平增加以及关键转录因子如与 runt 相关基因 2（Runx2）增加。
高亲水性支架可能会加速骨组织工程中的成骨分化。
