Yassin Mohammed A, Mustafa Kamal, Xing Zhe, Sun Yang, Fasmer Kristine Eldevik, Waag Thilo, Krueger Anke, Steinmüller-Nethl Doris, Finne-Wistrand Anna, Leknes Knut N
Department of Clinical Dentistry, Center for Clinical Dental Research Faculty of Medicine and Dentistry, University of Bergen, N-5020, Bergen, Norway.
Department of Clinical Science, Faculty of Medicine and Dentistry, University of Bergen, N-5020, Bergen, Norway.
Macromol Biosci. 2017 Jun;17(6). doi: 10.1002/mabi.201600427. Epub 2017 Jan 24.
Functionalizing polymer scaffolds with nanodiamond particles (nDPs) has pronounced effect on the surface properties, such as improved wettability, an increased active area and binding sites for cellular attachment and adhesion, and increased ability to immobilize biomolecules by physical adsorption. This study aims to evaluate the effect of poly(l-lactide-co-ε-caprolactone) (poly(LLA-co-CL)) scaffolds, functionalized with nDPs, on bone regeneration in a rat calvarial critical size defect. Poly(LLA-co-CL) scaffolds functionalized with nDPs are also compared with pristine scaffolds with reference to albumin adsorption and seeding efficiency of bone marrow stromal cells (BMSCs). Compared with pristine scaffolds, the experimental scaffolds exhibit a reduction in albumin adsorption and a significant increase in the seeding efficiency of BMSCs (p = 0.027). In the calvarial defects implanted with BMSC-seeded poly(LLA-co-CL)/nDPs scaffolds, live imaging at 12 weeks discloses a significant increase in osteogenic metabolic activity (p = 0.016). Microcomputed tomography, confirmed by histological data, reveals a substantial increase in bone volume (p = 0.021). The results show that compared with conventional poly(LLA-co-CL) scaffolds those functionalized with nDPs promote osteogenic metabolic activity and mineralization capacity. It is concluded that poly(LLA-co-CL) composite matrices functionalized with nDPs enhance osteoconductivity and therefore warrant further study as potential scaffolding material for bone tissue engineering.
用纳米金刚石颗粒（nDPs）对聚合物支架进行功能化处理，会对其表面性质产生显著影响，比如改善润湿性、增加细胞附着和黏附的活性面积及结合位点，以及提高通过物理吸附固定生物分子的能力。本研究旨在评估用nDPs功能化的聚（L-丙交酯-共-ε-己内酯）（聚（LLA-共-CL））支架对大鼠颅骨临界尺寸缺损骨再生的影响。还将用nDPs功能化的聚（LLA-共-CL）支架与原始支架在白蛋白吸附和骨髓间充质干细胞（BMSCs）接种效率方面进行比较。与原始支架相比，实验支架的白蛋白吸附减少，BMSCs接种效率显著提高（p = 0.027）。在植入接种了BMSCs的聚（LLA-共-CL）/nDPs支架的颅骨缺损处，12周时的活体成像显示成骨代谢活性显著增加（p = 0.016）。经组织学数据证实的微型计算机断层扫描显示骨体积大幅增加（p = 0.021）。结果表明，与传统的聚（LLA-共-CL）支架相比，用nDPs功能化的支架可促进成骨代谢活性和矿化能力。结论是，用nDPs功能化的聚（LLA-共-CL）复合基质增强了骨传导性，因此作为骨组织工程的潜在支架材料值得进一步研究。
