Sertkaya Zulfu, Koca Orhan, Ozturk Metin, Akyuz Mehmet, Gumrukcu Gulistan, Kutluhan Musab Ali, Karaman Muhammet Ihsan
Department of Urology, Memorial Hospital, Diyarbakir, Turkey.
Department of Urology, Medistate Hospital, Istanbul, Turkey.
Eurasian J Med. 2020 Jun;52(2):115-119. doi: 10.5152/eurasianjmed.2020.19229. Epub 2020 Jun 4.
Testicular torsion causes migration of neutrophils to the ischemic region and formation of free oxygen radicals that have a critical effect on ischemic reperfusion (I/R) injury. Udenafil is a selective, strong, and reversible inhibitor of phosphodiesterase type enzyme. In our study, we evaluate the protective effect of udenafil against reperfusion injury due to I/R.
Twenty-one male, adult, Wistar-Albino rats aged 8 months were randomly divided into three groups; sham, I/R, and I/R+udenafil. One hour before the detorsion operation, the sham and I/R groupssaline, and I/R+udenafil groups were administered 2 mg/kg udenafil intraperitoneally. Blood samples were collected to evaluate the inflammatory mediators. Spermatogenic factors were evaluated according to Johnsen criteria.
Histopathological and molecular parameters from all groups were compared. Mean values of TNF-α and IL-1β in venous blood samples were calculated. We observed that TNF-a values were statistically significantly increased in the I/R group than those in sham groups, and these values were decreased with udenafil treatment Furthermore, the glutathione peroxidase (GPx) level was statistically significantly decreased in the I/R group, and treatment with udenafil prevented this decrease. Evaluation of spermatogenesis using the Johnsen scoring system showed no statistically significant difference in mean scores between the groups.
We concluded that deterioration of biochemical and histopathological parameters are reversed, and injury due to I/R in testicle tissue may be decreased with udenafil treatment. Results of this experimental study show that efficacy of the udenafil treatment in testis torsion should be investigated.
睾丸扭转会导致中性粒细胞迁移至缺血区域，并形成对缺血再灌注（I/R）损伤具有关键影响的游离氧自由基。乌地那非是一种选择性、强效且可逆的磷酸二酯酶类型酶抑制剂。在我们的研究中，我们评估了乌地那非对I/R所致再灌注损伤的保护作用。
将21只8月龄的成年雄性Wistar - Albino大鼠随机分为三组；假手术组、I/R组和I/R + 乌地那非组。在扭转复位手术前1小时，假手术组和I/R组给予生理盐水，I/R + 乌地那非组腹腔注射2 mg/kg乌地那非。采集血样以评估炎症介质。根据约翰森标准评估生精因子。
比较了所有组的组织病理学和分子参数。计算静脉血样本中TNF-α和IL-1β的平均值。我们观察到，I/R组的TNF-α值在统计学上显著高于假手术组，而乌地那非治疗可降低这些值。此外，I/R组的谷胱甘肽过氧化物酶（GPx）水平在统计学上显著降低，乌地那非治疗可防止这种降低。使用约翰森评分系统评估精子发生，结果显示各组平均评分之间无统计学显著差异。
我们得出结论，生化和组织病理学参数的恶化可被逆转，乌地那非治疗可能会减少睾丸组织中I/R所致的损伤。本实验研究结果表明，应研究乌地那非治疗睾丸扭转的疗效。
