Beerstecher H J, Huiskens-van der Meij C, Warnaar S O
Department of Pathology, University of Leiden, The Netherlands.
J Histochem Cytochem. 1988 Sep;36(9):1153-60. doi: 10.1177/36.9.3261310.
We developed two monoclonal antibodies, E5 and F5, which react with mouse epidermal growth factor (mEGF) and urogastrone. These antibodies and a rabbit antiserum to mEGF were used for immunohistochemical analysis of staining reactions in rodent and human tissues. Our results do not confirm the published reports of EGF-like immunoreactivity in Brunner's glands, human submandibular glands, human kidney tissue, or rodent brain sections.
我们研制了两种单克隆抗体，E5和F5，它们可与小鼠表皮生长因子（mEGF）及urogastrone发生反应。这些抗体以及一种针对mEGF的兔抗血清被用于对啮齿动物和人类组织中的染色反应进行免疫组织化学分析。我们的结果并未证实已发表的有关在布伦纳腺、人类颌下腺、人类肾组织或啮齿动物脑切片中存在表皮生长因子样免疫反应性的报道。
