[Peroxidase immunohistochemical localization of the epidermal growth factor and/or urogastrone: 2 polypeptides present in the digestive tract].

Cytoplasmic localization of the antigen Epidermal Growth Factor (EGF) in Brunner's gland cells and in ductal submandibulary gland cells of rats has been effected by using the indirect immunoperoxidase antibody technique with an immunoserum obtained from highly purified extract of EGF/Urogastrone. After fixing in a buffered formaldehyde solution, the sections have been imbedded in paraffin. Structural and immunological properties have been well preserved by immunohistochemical specific techniques. These two polypeptides have similar biological activities and may therefore play an important role as therapeutic factors protectings against acid gastric secretion. An extensive revision and analysis of present stage of knowledge on these two substances which are active participants in the process of acid gastric secretion, has also been carried out.