小鼠精子获能过程中的代谢变化。
Metabolic changes in mouse sperm during capacitation†.
机构信息
Department of Pharmacology, Weill Cornell Medical College, New York, NY, USA.
Department of Veterinary and Animal Sciences, Integrated Science Building (ISB), University of Massachusetts, Amherst, MA, USA.
出版信息
Biol Reprod. 2020 Oct 5;103(4):791-801. doi: 10.1093/biolre/ioaa114.
Abstract
Mammalian sperm are stored in the epididymis in a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm modify their metabolism to meet this need. We now show that capacitating mouse sperm enhance glucose uptake, identifying glucose uptake as a functional marker of capacitation. Using an extracellular flux analyzer, we show that glycolysis and oxidative phosphorylation increase during capacitation. Furthermore, this increase in oxidative phosphorylation is dependent on glycolysis, providing experimental evidence for a link between glycolysis and oxidative phosphorylation in mouse sperm.
摘要
哺乳动物的精子在附睾中以休眠状态储存。在射精时，它们必须立即开始产生足够的能量来维持运动能力并支持获能。虽然获能过程中能量需求的增加是众所周知的，但目前尚不清楚小鼠精子如何改变其代谢以满足这一需求。我们现在表明，获能的小鼠精子增强了葡萄糖摄取，将葡萄糖摄取鉴定为获能的功能标志物。使用细胞外通量分析仪，我们表明在获能过程中糖酵解和氧化磷酸化增加。此外，这种氧化磷酸化的增加依赖于糖酵解，为小鼠精子中糖酵解和氧化磷酸化之间的联系提供了实验证据。
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本文引用的文献
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J Vis Exp. 2020 Jan 22(155). doi: 10.3791/60815.
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Shedding light on the role of cAMP in mammalian sperm physiology.揭示 cAMP 在哺乳动物精子生理学中的作用。
Mol Cell Endocrinol. 2018 Jun 15;468:111-120. doi: 10.1016/j.mce.2017.11.008. Epub 2017 Nov 13.
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The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm.酪氨酸激酶FER负责小鼠精子中与获能相关的酪氨酸磷酸化增加。
Development. 2016 Jul 1;143(13):2325-33. doi: 10.1242/dev.136499. Epub 2016 May 25.
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Expression, cellular localization, and involvement of the pentose phosphate pathway enzymes in the regulation of ram sperm capacitation.磷酸戊糖途径酶在公羊精子获能调节中的表达、细胞定位及作用
Theriogenology. 2016 Aug;86(3):704-14. doi: 10.1016/j.theriogenology.2016.02.024. Epub 2016 Mar 8.
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Differences in ATP Generation Via Glycolysis and Oxidative Phosphorylation and Relationships with Sperm Motility in Mouse Species.小鼠物种中通过糖酵解和氧化磷酸化产生ATP的差异及其与精子活力的关系。
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