Bekhit Mohamad, Abu El-Naga Mohamed N, Sokary Rehab, Fahim Ramy Amer, El-Sawy Naeem M
Radiation Chemistry Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2020;55(10):1210-1217. doi: 10.1080/10934529.2020.1784656. Epub 2020 Jul 2.
Recently, Silver nanoparticles (AgNPs) have become widely applied nanomaterial in human contacting areas such as cosmetics, food and medicine due to their antibacterial property. On the other hand, surfactants are essential ingredient of several industrial and consumer formulations. Based on these important applications, the current research was aimed to carry out the synthesis and characterization of Tween 80 capped silver nanoparticles (T80-AgNPs) using gamma radiation reduction method. Characterization of T80-AgNPs was occurred by using UV-Vis, XRD, FTIR and TEM techniques. UV-Visible spectra showed surface plasmon resonance (SPR) peak in the range of 420 nm signifying the synthesis of colloidal AgNPs. TEM confirmed the formation of spherical and uniformly distributed AgNPs with average size of 18 nm. XRD analysis illustrated the formation of pure crystalline AgNPs. The FTIR analysis provides evidence for the stabilization of AgNPs by Tween 80. The synthesized T80-AgNPs were evaluated for antibacterial activity against both ( as gram negative (G -ve) bacteria and () as gram positive (G + ve) bacteria and anti-biofilm activity to The results show that T80-AgNPs exhibits excellent antibacterial and antibiofilm activities.
近年来，由于具有抗菌特性，银纳米颗粒（AgNPs）已成为化妆品、食品和医药等人类接触领域中广泛应用的纳米材料。另一方面，表面活性剂是几种工业和消费配方中的重要成分。基于这些重要应用，当前的研究旨在采用γ射线还原法进行吐温80包覆银纳米颗粒（T80-AgNPs）的合成与表征。通过紫外可见光谱（UV-Vis）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和透射电子显微镜（TEM）技术对T80-AgNPs进行表征。紫外可见光谱显示表面等离子体共振（SPR）峰在420nm范围内，表明合成了胶体AgNPs。透射电子显微镜证实形成了平均尺寸为18nm的球形且均匀分布的AgNPs。X射线衍射分析表明形成了纯结晶AgNPs。傅里叶变换红外光谱分析为吐温80对AgNPs的稳定作用提供了证据。对合成的T80-AgNPs针对革兰氏阴性（G -ve）细菌和革兰氏阳性（G +ve）细菌的抗菌活性以及对生物膜的抗生物膜活性进行了评估。结果表明，T80-AgNPs表现出优异的抗菌和抗生物膜活性。
