Lublin University of Technology, Faculty of Environmental Engineering, Lublin, Poland.
University of Warmia and Mazury in Olsztyn, Faculty of Environmental Sciences, Olsztyn, Poland.
PLoS One. 2020 Jul 2;15(7):e0235508. doi: 10.1371/journal.pone.0235508. eCollection 2020.
This study examined the influence of bioaugmentation on metal concentrations (aluminum, cadmium, chromium, cobalt, copper, iron, lead, manganese, molybdenum, nickel and zinc) in anaerobically digested sewage sludge. To improve the digestion efficiency, bioaugmentation with a mixture of wild-living Archaea and Bacteria (MAB) from Yellowstone National Park, USA, was used. The total concentration of all metals was higher in the digestate than in the feedstock. During anaerobic digestion, the percent increase in the concentration of most of metals was slightly higher in the bioaugmented runs than in the un-augmented runs, but these differences were not statistically significant. However, the percent increase in cadmium and cobalt concentration was significantly higher in the bioaugmented runs than in the un-augmented runs. At MAB doses of 9 and 13% v/v, cadmium concentration in the digestate was 211 and 308% higher than in the feedstock, respectively, and cobalt concentration was 138 and 165%, respectively. Bioaugmentation increased over 4 times the percentage of Pseudomonas sp. in the biomass that are able to efficiently accumulate metals by both extracellular adsorption and intracellular uptake. Biogas production was not affected by the increased metal concentrations. In conclusion, bioaugmentation increased the concentration of metals in dry sludge, which means that it could potentially have negative effects on the environment.
本研究考察了生物强化对厌氧消化污水污泥中金属浓度（铝、镉、铬、钴、铜、铁、铅、锰、钼、镍和锌）的影响。为了提高消化效率，使用了来自美国黄石国家公园的野生古菌和细菌混合物（MAB）进行生物强化。与进料相比，消化产物中的所有金属的总浓度都更高。在厌氧消化过程中，大多数金属的浓度增加百分比在生物强化运行中略高于未强化运行，但这些差异没有统计学意义。然而，镉和钴浓度的增加百分比在生物强化运行中明显高于未强化运行。在 MAB 剂量为 9%和 13%（v/v）时，消化产物中镉的浓度分别比进料高出 211%和 308%，钴的浓度分别高出 138%和 165%。生物强化使能够通过细胞外吸附和细胞内摄取有效积累金属的假单胞菌属在生物量中的百分比增加了 4 倍以上。沼气产量不受金属浓度增加的影响。总之，生物强化增加了干污泥中金属的浓度，这意味着它可能对环境产生负面影响。
