Raza Sayed Haidar Abbas, Gui Linsheng, Khan Rajwali, Schreurs Nicola M, Xiaoyu Wang, Wu Sen, Mei Chugang, Wang Li, Ma Xueyao, Wei Dawei, Guo Hongfang, Zhang Song, Wang Xingping, Kaleri Hubdar Ali, Zan Linsen
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China; National Beef Cattle Improvement Center of Northwest A&F University, Yangling 712100, PR China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai 810016, People's Republic of China.
Gene. 2018 Mar 1;645:55-59. doi: 10.1016/j.gene.2017.12.034. Epub 2017 Dec 19.
Fatty acid synthase (FASN) is an enzyme involved with fat deposition and fatty acid composition in cattle. This study was conducted to detect single nucleotide polymorphisms (SNPs) of the FASN gene and explore their relationships with ultrasound carcass traits in order to assess the potential use of the FASN gene for the breeding selection of Qinchuan cattle for desirable carcass traits. The frequencies of SNP g.12740C>T, g.13192T>C and g.13232C>T were identified in 525 individual Qinchuan cattle which were also assessed for backfat depth, eye muscle area and intramuscular fat by ultrasound. According to the PIC values, g.13192T>C possessed an intermediate polymorphism (0.25<PIC<0.5). The SNPs of g.13232C>T, g.12740C>T possessed low polymorphism (PIC<0.25). Chi-square tests showed that g.13192T>C were in Hardy-Weinberg disequilibrium (c2<c0.052). Two SNPs were found to be associated with variation in ultrasound carcass traits. The HH diplotypes had a greater back fat depth than HH, HH and HH (P<0.01). The TT genotype at g.13192T>C was associated with a greater eye muscle area and the TT genotype at g.13232C>T was associated with greater intramuscular fat. When these genotypes were combined there was no difference in eye muscle area and intramuscular fat between the diplotypes. The HH diplotype was associated with carcass traits that are likely to provide economic advantage in Qinchuan cattle. Variations in the FASN genes and their corresponding genotypes may be considered as molecular markers for economic traits in cattle breeding.
脂肪酸合酶（FASN）是一种与牛脂肪沉积和脂肪酸组成相关的酶。本研究旨在检测FASN基因的单核苷酸多态性（SNP），并探索其与超声胴体性状的关系，以评估FASN基因在秦川牛理想胴体性状选育中的潜在应用价值。在525头秦川牛个体中鉴定了SNP g.12740C>T、g.13192T>C和g.13232C>T的频率，同时通过超声对这些牛的背膘厚度、眼肌面积和肌内脂肪进行了评估。根据PIC值，g.13192T>C具有中度多态性（0.25<PIC<0.5）。g.13232C>T、g.12740C>T的SNP具有低度多态性（PIC<0.25）。卡方检验表明，g.13192T>C处于哈迪-温伯格不平衡状态（c2<c0.052）。发现两个SNP与超声胴体性状的变异有关。HH单倍型的背膘厚度大于HH、HH和HH（P<0.01）。g.13192T>C处的TT基因型与更大眼肌面积相关，g.13232C>T处的TT基因型与更多肌内脂肪相关。当这些基因型组合时，单倍型之间的眼肌面积和肌内脂肪没有差异。HH单倍型与可能为秦川牛带来经济优势的胴体性状相关。FASN基因的变异及其相应的基因型可被视为牛育种中经济性状的分子标记。
