Division of Plastic Surgery, Zhongshan Hospital Xiamen University, Xiamen, PR China.
Biotechcomer (Xiamen) Science and Technology Ltd, Xiamen, PR China.
J Plast Surg Hand Surg. 2020 Dec;54(6):344-351. doi: 10.1080/2000656X.2020.1781138. Epub 2020 Jul 2.
Nanofat grafting is a fat transfer procedure that uses a thin needle to smooth out wrinkles, thereby achieving the goal of skin rejuvenation. The Luer-Lok connector is one of the most common methods for obtaining Nanofat. In the present study, we compared three different Luer-Lok connectors (2.0 mm, 1.5 mm and 1.1 mm in diameter) in terms of their impact on the viability of adipose-derived stem cells (ADSCs) to determine the optimal size of the connector for efficient Nanofat grafting. We observed that a smaller diameter of the Luer-Lok connector created a higher mechanical shear force, which broke more fat cells during the emulsifying procedure, thereby reducing the viability of ADSCs from the stromal vascular fraction (SVF). Nanofat obtained from the 2-mm Luer-Lok connector had a better effect on skin rejuvenation than the 1.5-mm and 1.1-mm connectors. Therefore, this study presents an advance in the simple procedure of preparing Nanofat based on a previous technique and provides evidence that a procedure associated with less trauma may be a better choice.
纳米脂肪移植是一种脂肪转移程序，使用细针来抚平皱纹，从而达到皮肤年轻化的目的。鲁尔锁接头是获取纳米脂肪最常用的方法之一。在本研究中，我们比较了三种不同直径的鲁尔锁接头（2.0mm、1.5mm 和 1.1mm）对脂肪来源干细胞（ADSCs）活力的影响，以确定用于高效纳米脂肪移植的最佳接头尺寸。我们观察到，直径较小的鲁尔锁接头会产生更高的机械剪切力，在乳化过程中会破坏更多的脂肪细胞，从而降低基质血管部分（SVF）中 ADSCs 的活力。与 1.5mm 和 1.1mm 接头相比，来自 2mm 鲁尔锁接头的纳米脂肪在皮肤年轻化方面的效果更好。因此，本研究在先前技术的基础上提出了一种简单的纳米脂肪制备方法的改进，为创伤较小的程序可能是更好的选择提供了证据。
