Che Dehui, Zhou Yongting, Wang Jie, Liu Ying, Gao Feng, Lv Tianqi, Cui Chenyang, Xiao Zhibo
Department of Plastic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
Department of Plastic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
J Plast Reconstr Aesthet Surg. 2022 Sep;75(9):3595-3602. doi: 10.1016/j.bjps.2022.04.055. Epub 2022 Apr 30.
Nanofat, as a derivative of adipose tissue, has gradually become a research hotspot in beauty and regenerative medicine. However, the nanofat preparation method has not yet been standardized; it remains unknown whether the aperture of the connector has any influence on the transplantation effect.
Adipose tissue was mechanically emulsified into nanofat tissue through different connector apertures (1.0, 1.5, and 2.0 mm). Cell survival and apoptosis were measured using the volume of oil droplets, glucose transportation test, flow cytometry, cell counting kit-8 (CCK-8), wound healing assay, transwell migration assay, and fluorescence staining. The expression of adiponectin, GluT4, and PPAR-γ in nanofat-derived stem cells (NFSCs) was detected using quantitative real-time polymerase chain reaction (qRT-PCR).
The fineness of nanofat tissue texture decreased with an increase in the aperture connector. The amounts of glucose transferred in the three groups (1, 1.5, and 2 mm) were 4.7 ± 0.894, 6.1 ± 1.026, and 6.9 ± 0.868 mmol/L, respectively. Flow cytometric analysis showed that the proportion of NFSCs in the 2.0 mm group was the highest (91.267±1.210%). Cell proliferation and migration abilities were stronger in the 1.5 and 2.0 mm groups. The numbers of late apoptotic and dead cells in the 2.0 mm group were significantly fewer than those in the two other groups. Expression levels of lipid-related genes were as follows: adiponectin > GluT4 > PPAR-γ in each component.
As nanofat is emulsified, the use of larger aperture connectors (2.0 mm) appeared to decrease the degree of adipocyte lysis and increase the biological activity of adipose tissue.
纳米脂肪作为脂肪组织的衍生物，已逐渐成为美容和再生医学领域的研究热点。然而，纳米脂肪的制备方法尚未标准化；连接器的孔径是否会对移植效果产生影响仍不清楚。
通过不同孔径（1.0、1.5和2.0毫米）的连接器将脂肪组织机械乳化制成纳米脂肪组织。使用油滴体积、葡萄糖转运试验、流式细胞术、细胞计数试剂盒-8（CCK-8）、伤口愈合试验、Transwell迁移试验和荧光染色来测量细胞存活和凋亡情况。采用定量实时聚合酶链反应（qRT-PCR）检测纳米脂肪来源干细胞（NFSCs）中脂联素、葡萄糖转运蛋白4（GluT4）和过氧化物酶体增殖物激活受体γ（PPAR-γ）的表达。
纳米脂肪组织质地的细腻程度随连接器孔径的增大而降低。三组（1、1.5和2毫米）葡萄糖转运量分别为4.7±0.894、6.1±1.026和6.9±0.868毫摩尔/升。流式细胞术分析显示，2.0毫米组中NFSCs的比例最高（91.267±1.210%）。1.5和2.0毫米组的细胞增殖和迁移能力更强。2.0毫米组晚期凋亡和死亡细胞的数量明显少于其他两组。各组分中脂质相关基因的表达水平如下：脂联素>葡萄糖转运蛋白4>过氧化物酶体增殖物激活受体γ。
随着纳米脂肪的乳化，使用较大孔径的连接器（2.0毫米）似乎可降低脂肪细胞的裂解程度，并提高脂肪组织的生物活性。
