CSIR-Central Electrochemical Research Institute (CSIR-CECRI), Karaikudi, Tamil Nadu 630006, India.
Indian Institute of Technology Madras, Chennai, India.
Biomed Mater. 2020 Oct 13;15(6):065019. doi: 10.1088/1748-605X/aba23a.
Surface-modified commercially pure titanium (Cp-Ti) with zirconium (Zr)-based thin film metallic glasses (Zr-TFMGs) and ZrO thin films were surgically implanted into the tibiae of rats; the bone formation was analyzed to examine the performance of the coatings as a biomaterial. Zr-TFMGs and ZrO thin films were coated on Cp-Ti substrates to monitor the control of assimilation in vitro and in vivo. The microstructural and elemental analyses were carried out for the as deposited thin films by x-ray diffraction (XRD), transmission electron microscopy and x-ray photoelectron spectroscopy. TFMG- and ZrO-coated Ti specimens were immersed in simulated body fluid (SBF) for a period of 21 days to evaluate the calcium phosphate precipitation in vitro. XRD, x-ray photoelectron spectroscopy and scanning electron microscopy/energy dispersive x-ray spectroscopy were used to quantify the mineralization on the coated Zr-TFMG and ZrO. In vitro corrosion studies showed that the Zr-based TFMG and ZrO coatings sustained in the SBF, exhibited superior corrosion resistance to the bare crystalline Ti substrate. Wettability studies showed TFMG and ZrO coatings with a hydrophobic nature, and the TFMG-coated SBF-submerged specimens showed a hydrophilic nature. The in vitro cell viability of MC3T3-E1 cells showed good cell proliferation and low cytotoxicity. The calcification deposits were evaluated by staining with alizarin red S, which showed a lower calcium formation on Zr-TFMG compared to ZrO. The present work also aims to assess the assimilation behavior of Cp-Ti, Zr-TFMG and ZrO in vivo by inserting the coated specimen in the femur of rats. After post-implantation of 8 weeks, specimens were examined by micro-CT evaluation. The bone contact ratios as calculated were 72.75%, 15.32% and 38.79%. Consequently, the bone affinity was Cp-Ti wire >ZrO-coated Ti wire >ZrCuAgAl-coated Ti wire.
表面改性商用纯钛（Cp-Ti）与锆（Zr）基薄膜金属玻璃（Zr-TFMG）和 ZrO 薄膜被手术植入大鼠胫骨中；分析骨形成以检查涂层作为生物材料的性能。Zr-TFMG 和 ZrO 薄膜涂覆在 Cp-Ti 基底上，以监测体外和体内的同化控制。通过 X 射线衍射（XRD）、透射电子显微镜和 X 射线光电子能谱对沉积的薄膜进行了微观结构和元素分析。将 TFMG 和 ZrO 涂层的 Ti 试样浸入模拟体液（SBF）中 21 天，以评估体外磷酸钙沉淀。XRD、X 射线光电子能谱和扫描电子显微镜/能谱用于定量测量涂层 Zr-TFMG 和 ZrO 上的矿化。体外腐蚀研究表明，Zr 基 TFMG 和 ZrO 涂层在 SBF 中能维持，表现出比裸露的晶态 Ti 基底更好的耐腐蚀性。润湿性研究表明 TFMG 和 ZrO 涂层具有疏水性，而 TFMG 涂层的 SBF 浸泡试样表现出亲水性。MC3T3-E1 细胞的体外细胞活力显示出良好的细胞增殖和低细胞毒性。通过茜素红 S 染色评估钙化沉积物，结果表明 Zr-TFMG 上的钙形成低于 ZrO。本工作还旨在通过将涂覆的试样插入大鼠股骨中来评估 Cp-Ti、Zr-TFMG 和 ZrO 的体内同化行为。植入 8 周后，通过微 CT 评估对试样进行了检查。计算出的骨接触比分别为 72.75%、15.32%和 38.79%。因此，骨亲和力为 Cp-Ti 丝＞ZrO 涂层 Ti 丝＞ZrCuAgAl 涂层 Ti 丝。
