Korba B E, Cote P J, Gerin J L
Division of Molecular Virology and Immunology, Georgetown University Medical Center, Rockville, MD 20852.
Science. 1988 Sep 2;241(4870):1213-6. doi: 10.1126/science.3261887.
Peripheral blood lymphocytes (PBLs) isolated from woodchucks chronically infected with the woodchuck hepatitis virus (WHV) carry low levels of nonreplicating WHV DNA. When PBLs from chronic carrier woodchucks were activated in culture with the generalized mitogen lipopolysaccharide (LPS), WHV DNA replication was initiated in cells obtained from one of three animals examined. Intracellular WHV core particles, containing WHV DNA replication intermediates, RNA/DNA hybrid molecules, and an active endogenous DNA polymerase, appeared 3 days after the start of LPS stimulation. After 5 to 7 days of LPS stimulation, WHV DNA-containing particles, which displayed the properties of intact, mature virions, were released into the culture medium. These studies provide evidence for reactivation of a latent WHV infection of circulating lymphoid cells and indicate that the presence of nonreplicating hepadnaviral DNA in lymphoid cells represents a potentially active infection following cellular activation.
从慢性感染土拨鼠肝炎病毒（WHV）的土拨鼠中分离出的外周血淋巴细胞（PBLs）携带低水平的非复制型WHV DNA。当用通用丝裂原脂多糖（LPS）在培养中激活慢性携带土拨鼠的PBLs时，在检测的三只动物中的一只所获得的细胞中启动了WHV DNA复制。含有WHV DNA复制中间体、RNA/DNA杂交分子和活性内源性DNA聚合酶的细胞内WHV核心颗粒在LPS刺激开始后3天出现。LPS刺激5至7天后，显示完整、成熟病毒体特性的含WHV DNA颗粒被释放到培养基中。这些研究为循环淋巴细胞中潜伏的WHV感染的重新激活提供了证据，并表明淋巴细胞中非复制型嗜肝DNA病毒DNA的存在代表细胞激活后潜在的活跃感染。
