Department of Environmental and Plant Biology, Ohio University, Athens, OH, USA.
Department of Biological Sciences, Kent State University, Kent, OH, USA.
J Appl Microbiol. 2021 Jan;130(1):109-122. doi: 10.1111/jam.14761. Epub 2020 Jul 16.
The soil microbial community plays a critical role in increasing phosphorus (P) availability in low-P, weathered soils by "mining" recalcitrant organic P through the production of phosphatase enzymes. However, there is a lack of data on the fungal and bacterial taxa which are directly involved in P mining, which could also serve as potential microbial bioindicators of low P availability.
Leveraging a 5-year P enrichment experiment on low-P forest soils, high-throughput sequencing was used to profile the microbial community to determine which taxa associate closely with P availability. We hypothesized that there would be a specialized group of soil micro-organisms that could access recalcitrant P and whose presence could serve as a bioindicator of P mining. Community profiling revealed several candidate bioindicators of P mining (Russulales, Acidobacteria Subgroup 2, Acidobacteriales, Obscuribacterales and Solibacterales), whose relative abundance declined with elevated P and had a significant, positive association with phosphatase production. In addition, we identified candidate bioindicators of high P availability (Mytilinidales, Sebacinales, Chitinophagales, Cytophagales, Saccharimonadales, Opitulales and Gemmatales).
This research provides evidence that mitigating P limitation in this ecosystem may be a specialized trait and is mediated by a few microbial taxa.
Here, we characterize Orders of soil microbes associated with manipulated phosphorus availability in forest soils to determine bioindicator candidates for phosphorus. Likewise, we provide evidence that the microbial trait to utilize recalcitrant organic forms of P (e.g. P mining) is likely a specialized trait and not common to all members of the soil microbial community. This work further elucidates the role that a complex microbial community plays in the cycling of P in low-P soils, and provides evidence for future studies on microbial linkages to human-induced ecosystem changes.
土壤微生物群落通过产生磷酸酶来“开采”难溶性有机磷，从而在低磷、风化土壤中增加磷的有效性，因此在增加低磷、风化土壤中的磷有效性方面起着关键作用。然而，直接参与磷矿开采的真菌和细菌类群的数据很少，这些类群也可以作为低磷可用性的潜在微生物生物指标。
利用一项为期 5 年的低磷森林土壤磷富集实验，利用高通量测序对微生物群落进行了分析，以确定与磷有效性密切相关的分类群。我们假设，可能存在一组专门的土壤微生物，可以利用难溶性磷，其存在可以作为磷矿开采的生物指标。群落分析揭示了一些磷矿开采的候选生物指标（Russulales、Acidobacteria Subgroup 2、Acidobacteriales、Obscuribacterales 和 Solibacterales），其相对丰度随着磷的增加而下降，与磷酸酶的产生呈显著正相关。此外，我们还确定了高磷可用性的候选生物指标（Mytilinidales、Sebacinales、Chitinophagales、Cytophagales、Saccharimonadales、Opitulales 和 Gemmatales）。
这项研究提供了证据表明，缓解该生态系统中的磷限制可能是一种专门的特征，由少数微生物类群介导。
在这里，我们描述了与森林土壤中人为控制的磷有效性相关的土壤微生物目，以确定磷的生物指标候选物。同样，我们提供的证据表明，利用难溶性有机磷形式的微生物特性（例如磷矿开采）可能是一种专门的特征，而不是所有土壤微生物群落成员所共有的。这项工作进一步阐明了复杂微生物群落在低磷土壤中磷循环中的作用，并为未来研究微生物与人为引起的生态系统变化的联系提供了证据。
