Moshage H J, Roelofs H M, van Pelt J F, Hazenberg B P, van Leeuwen M A, Limburg P C, Aarden L A, Yap S H
Department of Medicine, St. Radboud University Hospital, Nijmegen, The Netherlands.
Biochem Biophys Res Commun. 1988 Aug 30;155(1):112-7. doi: 10.1016/s0006-291x(88)81056-8.
During the acute phase response, synthesis of C-reactive protein and serum amyloid A is increased. To investigate whether the enhanced synthesis of these proteins are due to stimulatory effect of inflammatory mediators such as interleukin-1 (IL-1) and interleukin-6 (IL-6) produced by macrophages and monocytes, primary cultures of adult human hepatocytes were exposed to recombinant (r)IL-1, rIL-6 or rIL-1 and monospecific anti rIL-6 antibodies in the presence of 1 microM dexamethasone. The findings indicate that rIL-1 and rIL-6 both stimulate the liver synthesis of C-reactive protein and serum amyloid A, however monospecific anti rIL-6 antibodies reduce the stimulatory effect of rIL-1 on the synthesis of these proteins. These findings suggest that IL-6 plays a key role in the stimulation of synthesis of serum amyloid A and C-reactive protein by the human liver cells.
在急性期反应期间，C反应蛋白和血清淀粉样蛋白A的合成增加。为了研究这些蛋白质合成的增强是否归因于巨噬细胞和单核细胞产生的炎性介质如白细胞介素-1（IL-1）和白细胞介素-6（IL-6）的刺激作用，将成人原代肝细胞培养物在1微摩尔地塞米松存在的情况下暴露于重组（r）IL-1、rIL-6或rIL-1和单特异性抗rIL-6抗体。研究结果表明，rIL-1和rIL-6均刺激肝脏合成C反应蛋白和血清淀粉样蛋白A，然而单特异性抗rIL-6抗体降低了rIL-1对这些蛋白质合成的刺激作用。这些发现提示，IL-6在人肝细胞刺激血清淀粉样蛋白A和C反应蛋白合成中起关键作用。
