Burstein Y, Buchner V, Pecht M, Trainin N
Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel.
Biochemistry. 1988 May 31;27(11):4066-71. doi: 10.1021/bi00411a024.
Thymic humoral factor gamma 2 (THF-gamma 2), an octapeptide essential for immune regulation, was purified from calf thymus. The purification of THF-gamma 2, monitored in vitro and in vivo in mouse splenocyte proliferation assays, was achieved by gel filtration of low molecular weight thymus extracts followed by ion-exchange chromatography and sequential reversed-phase high-performance liquid chromatography. The process yielded 5 micrograms of THF-gamma 2/1000 kg of thymus tissue. The concentration of THF-gamma 2 required for augmentation of lymphocyte proliferation and interleukin 2 production was 5 ng/mL in vitro and 10 ng/kg per mouse in vivo. THF-gamma 2 has the amino acid sequence Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu. The proposed structure has been confirmed because a peptide was synthesized on the basis of this sequence that showed activity identical with that of the biological molecule. It shows no homology to the amino acid sequence of other thymic hormones nor is it part of any peptide or protein of known sequence. THF-gamma 2 retains essentially all of the biological activity of the thymus extract from which it is derived.
胸腺体液因子γ2（THF-γ2）是一种对免疫调节至关重要的八肽，从牛胸腺中纯化得到。在小鼠脾细胞增殖试验中通过体外和体内监测来纯化THF-γ2，方法是先对低分子量胸腺提取物进行凝胶过滤，然后进行离子交换色谱和连续反相高效液相色谱。该过程每1000千克胸腺组织可产生5微克THF-γ2。体外增强淋巴细胞增殖和白细胞介素2产生所需的THF-γ2浓度为5纳克/毫升，体内为每只小鼠10纳克/千克。THF-γ2的氨基酸序列为Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu。所提出的结构已得到证实，因为根据该序列合成的一种肽显示出与生物分子相同的活性。它与其他胸腺激素的氨基酸序列没有同源性，也不是任何已知序列的肽或蛋白质的一部分。THF-γ2基本上保留了其来源的胸腺提取物的所有生物活性。
