Dardenne M, Pleau J M, Man N K, Bach J F
J Biol Chem. 1977 Nov 25;252(22):8040-4.
A circulating thymic factor (FTS) has been characterized by a bioassay based on its ability to render theta-negative rosette-forming cells theta-positive and azathioprine-sensitive. FTS was sequentially purified and finally isolated from 1000 liters of pig serum by ultrafiltration, gel filtration, and ion exchange chromatography. Its amino acid composition and apparent molecular weight estimated by Sephadex G-25 chromatography, indicate that FTS is a nonapeptide of composition lysine, aspartic acid (or asparagine), serine 2, glutamic acid (or glutamine) 2, glycine 2, and alanine.
一种循环胸腺因子（FTS）已通过一种生物测定法得以鉴定，该生物测定法基于其使θ阴性玫瑰花结形成细胞变为θ阳性且对硫唑嘌呤敏感的能力。FTS经过了一系列纯化步骤，最终通过超滤、凝胶过滤和离子交换色谱法从1000升猪血清中分离出来。通过Sephadex G - 25色谱法估计其氨基酸组成和表观分子量，结果表明FTS是一种由赖氨酸、天冬氨酸（或天冬酰胺）、2个丝氨酸、2个谷氨酸（或谷氨酰胺）、2个甘氨酸和丙氨酸组成的九肽。
