Division of Laboratory, Yamaguchi University Hospital, Ube, Japan.
Division of Laboratory, Yamaguchi University Hospital, Ube, Japan.
Clin Chim Acta. 2020 Nov;510:15-20. doi: 10.1016/j.cca.2020.06.046. Epub 2020 Jul 2.
Hemolysis is a common problem in the handling of serum specimens. The hemolysis index (HI) provides a warning of hemolysis in auto-analyzers. However, HI has not been standardized, and each laboratory's original method is applied. Especially, the wavelength used for HI measurement is different in each laboratory. Thus, we investigated the warning ability of HI at various wavelengths.
We selected 4 wavelength types, and each HI was measured and calculated (410 nm/HI-1, 451 nm/HI-2, 545 nm/HI-3, and 571 nm/HI-4). To compare the 4 HI types, we investigated the influence of 3 interference components using artificially hemolyzed specimens (AHSs). We also investigated both the relationship between HI and hemoglobin concentration (Hb) and that between HI and 31 biochemical test values in AHSs.
In the interference assessment, only HI-4 showed no influence on the 3 interference components. The correlation between Hb and HI-4 was very strong (r = 0.9987). A 1-unit increase in HI-4 corresponded to a 14.8-mg/dL increase in Hb.
We found the best wavelength for HI to be at or near 571 nm.
溶血是血清标本处理中常见的问题。溶血指数（HI）为自动分析仪溶血提供了警示。然而，HI 尚未标准化，每个实验室都采用原始方法。特别是，每个实验室用于 HI 测量的波长都不同。因此，我们研究了不同波长下 HI 的警示能力。
我们选择了 4 种波长类型，分别测量和计算每个 HI（410nm/HI-1、451nm/HI-2、545nm/HI-3 和 571nm/HI-4）。为了比较 4 种 HI 类型，我们使用人工溶血标本（AHS）研究了 3 种干扰成分的影响。我们还研究了 HI 与血红蛋白浓度（Hb）之间的关系以及 HI 与 AHS 中 31 项生化测试值之间的关系。
在干扰评估中，只有 HI-4 对 3 种干扰成分没有影响。Hb 与 HI-4 之间的相关性非常强（r=0.9987）。HI-4 增加 1 单位，Hb 增加 14.8mg/dL。
我们发现 HI 的最佳波长在 571nm 左右或附近。
