Ishiguro Akiyo, Nishioka Mitsuaki, Morishige Akihiro, Yoneshiro Mai, Shinkawa Kanae, Fujinaga Aki, Kobayashi Toshihiko, Suehiro Yutaka, Yamasaki Takahiro
Department of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan.
Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan.
J Clin Med. 2023 Sep 9;12(18):5864. doi: 10.3390/jcm12185864.
Many biochemical auto-analyzers have methods that measure the hemolysis index (HI) to quantitatively assess the degree of hemolysis. Past reports on HI are mostly in vitro studies. Therefore, we evaluated the optimal wavelength of HI measurement ex vivo using clinical samples. Four different wavelengths (410/451 nm: HI-1, 451/478 nm: HI-2, 545/596 nm: HI-3 and 571/596 nm: HI-4) were selected for HI measurement, and correlations were examined from the measurement results of 3890 clinical samples. Another set of 9446 clinical samples was used to examine the correlation of HI with lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and potassium (K). Strong correlations were found between HI-4 and HI-1 and between HI-4 and HI-3. HI-1 and HI-2 cannot correctly assess hemolysis for high bilirubin samples, and HI-3 cannot correctly assess hemolysis for high triglyceride samples. LDH, AST and K correlated positively with HI-4 in clinical samples. For every 1-unit increase in HI-4, LDH increased by 19.51 U/L, AST by 1.03 U/L and K by 0.061 mmol/L, comparable to reports of other studies. In clinical samples, HI-4 was less susceptible to bilirubin and chyle and reflected well the changes in LDH, AST and K caused by hemolysis. This suggested that the optimal wavelength for HI measurement is 571 nm.
许多生化自动分析仪都有测量溶血指数（HI）以定量评估溶血程度的方法。过去关于HI的报告大多是体外研究。因此，我们使用临床样本对离体测量HI的最佳波长进行了评估。选择了四个不同的波长（410/451nm：HI-1、451/478nm：HI-2、545/596nm：HI-3和571/596nm：HI-4）进行HI测量，并从3890份临床样本的测量结果中检查相关性。另一组9446份临床样本用于检查HI与乳酸脱氢酶（LDH）、天冬氨酸转氨酶（AST）和钾（K）的相关性。发现HI-4与HI-1之间以及HI-4与HI-3之间存在强相关性。HI-1和HI-2不能正确评估高胆红素样本的溶血情况，HI-3不能正确评估高甘油三酯样本的溶血情况。临床样本中，LDH、AST和K与HI-4呈正相关。HI-4每增加1个单位，LDH增加19.51U/L，AST增加1.03U/L，K增加0.061mmol/L，与其他研究报告相当。在临床样本中，HI-4受胆红素和乳糜的影响较小，能很好地反映溶血引起的LDH、AST和K的变化。这表明测量HI的最佳波长为571nm。
