Medical Care Clinic, Beijing Luhe Hospital, Capital Medical University, Beijing, China.
Department of Rehabilitation, Beijing Rehabilitation Hospital, Capital Medical University, Beijing, China.
Exp Mol Pathol. 2020 Oct;116:104489. doi: 10.1016/j.yexmp.2020.104489. Epub 2020 Jul 2.
To uncover the role of microRNA-487a-3p (miR-487a-3p) in influencing the malignant development of pancreatic cancer and the involvement of its downstream target SMAD7.
MiR-487a-3p level in 40 pancreatic cancer and paracancerous tissues was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between miR-487a-3p level and clinical indicators in pancreatic cancer patients was analyzed. Regulatory effects of miR-487a-3p on biological phenotypes of pancreatic cancer cells were assessed. At last, the involvement of miR-487a-3p and its downstream target SMAD7 in pancreatic cancer was determined.
MiR-487a-3p was lowly expressed in pancreatic cancer tissues. Pancreatic cancer patients expressing a low level of miR-487a-3p suffered high metastasis rate and poor prognosis. Overexpression of miR-487a-3p markedly attenuated proliferative and migratory capacities in pancreatic cancer cells. SMAD7 was the downstream target of miR-487a-3p, which was highly expressed in pancreatic cancer samples. Overexpression of SMAD7 reversed the regulatory effects of miR-487a-3p on pancreatic cancer cell phenotypes.
MiR-487a-3p is downregulated in pancreatic cancer samples, which is linked to metastasis and prognosis in pancreatic cancer. It inhibits the malignant development of pancreatic cancer by negatively regulating SMAD7.
揭示微小 RNA-487a-3p（miR-487a-3p）在影响胰腺癌恶性发展及其下游靶基因 SMAD7 中的作用。
采用实时定量聚合酶链反应（qRT-PCR）检测 40 例胰腺癌及癌旁组织中 miR-487a-3p 的水平。分析 miR-487a-3p 水平与胰腺癌患者临床指标的关系。评估 miR-487a-3p 对胰腺癌细胞生物学表型的调控作用。最后，确定 miR-487a-3p 及其下游靶基因 SMAD7 在胰腺癌中的作用。
miR-487a-3p 在胰腺癌组织中低表达。miR-487a-3p 低表达的胰腺癌患者转移率高，预后差。miR-487a-3p 的过表达显著减弱了胰腺癌细胞的增殖和迁移能力。SMAD7 是 miR-487a-3p 的下游靶基因，在胰腺癌样本中高表达。SMAD7 的过表达逆转了 miR-487a-3p 对胰腺癌细胞表型的调节作用。
miR-487a-3p 在胰腺癌样本中下调，与胰腺癌的转移和预后有关。它通过负调控 SMAD7 抑制胰腺癌的恶性发展。
