三种氟化物化合物在增加微观生物膜成熟度方面的抗龋效果。

The anti-caries efficacy of three fluoride compounds at increasing maturation of a microcosm biofilm.

机构信息

King Saud University, College of Applied Medical Sciences, Dental Health Department, P.O. Box 145111, Riyadh, 4545, Saudi Arabia; Indiana University, School of Dentistry, Department of Biomedical Sciences and Comprehensive Care, 1121 W. Michigan Street, Indianapolis, IN, 46202, USA; Indiana University, School of Medicine, Department of Family Medicine, Bowen Center for Health Workforce Research & Policy, 1110 W. Michigan Street, Indianapolis, IN, 46202, USA.

Indiana University, School of Dentistry, Department of Biomedical Sciences and Comprehensive Care, 1121 W. Michigan Street, Indianapolis, IN, 46202, USA.

出版信息

Arch Oral Biol. 2020 Sep;117:104781. doi: 10.1016/j.archoralbio.2020.104781. Epub 2020 May 30.

DOI:10.1016/j.archoralbio.2020.104781

PMID:32622258

Abstract

OBJECTIVE

To explore the anti-caries efficacy of three fluoride compounds at increasing maturation of a microcosm biofilm.

DESIGN

Microcosm biofilm, obtained from saliva collected from three donors (IRB #1406440799), was grown on enamel samples (n = 18/group) for 24-h (Brain Heart Infusion; 0.2 % sucrose). Then, pH cycling model started. Three maturations were explored (4d, 8d, and 12d). The pH cycling consisted of daily 2 × 5 min treatments (NaF, SnF, AmF: 287.5 ppm F, and de-ionized water [DIW]), 4 × 10 min remineralization (BHI, no sucrose, pH 7.0), and 3 × 2:15 h demineralization (BHI, 1% sucrose, pH 4.5). We analyzed the enamel (surface microhardness [VHN], integrated mineral loss [ΔZ], lesion depth [L]), and the biofilm (viability [log CFU/mL], lactic acid production [LDH], and exopolysaccharide [EPS] amount). Data were analyzed using two-way ANOVA (p = 0.05).

RESULTS

The interaction between tested variables was significant for VHN, viability, LDH, EPS (p = 0.0354, p = 0.0001, p < 0.0001, p < 0.0001), but not for L (p = 0.2412) or ΔZ (p = 0.6811). LDH and EPS analyses exhibited more tolerance of mature biofilm against NaF (LDH and EPS p < 0.0001); NaF-treated groups demonstrated significantly lower results than the control in the 12d group. The effect of SnF and AmF continued over time. VHN, L, and ΔZ: The effect of SnF and AmF was higher than NaF and DIW. L and ΔZ did not result in significant differences over time (all treatments). Within each maturation, fluoride compounds demonstrated statistically significantly lower L and ΔZ values than DIW.

CONCLUSIONS

Biofilm's maturation may influence the selection of fluoride compounds to achieve an optimum cariostatic effect.

摘要

目的

研究三种氟化物化合物在增加微宇宙生物膜成熟度方面的抗龋效果。

设计

从三名供体（IRB#1406440799）采集的唾液中获得微宇宙生物膜，在釉质样本上培养 24 小时（Brain Heart Infusion；0.2%蔗糖）。然后，开始 pH 循环模型。探索了三种成熟度（4d、8d 和 12d）。pH 循环包括每日 2×5min 处理（NaF、SnF、AmF：287.5ppmF 和去离子水[DIW]）、4×10min 再矿化（BHI，无蔗糖，pH7.0）和 3×2:15h 脱矿化（BHI，1%蔗糖，pH4.5）。我们分析了釉质（表面显微硬度[VHN]、整体矿物质损失[ΔZ]、病变深度[L]）和生物膜（活力[log CFU/mL]、乳酸产量[LDH]和胞外多糖[EPS]量）。使用双向方差分析（p=0.05）分析数据。

结果

测试变量之间的相互作用对 VHN、活力、LDH、EPS 具有显著性（p=0.0354，p=0.0001，p<0.0001，p<0.0001），但对 L（p=0.2412）或ΔZ（p=0.6811）没有显著性。LDH 和 EPS 分析显示成熟生物膜对 NaF 的耐受性更强（LDH 和 EPS p<0.0001）；在 12d 组中，NaF 处理组的结果明显低于对照组。SnF 和 AmF 的作用持续时间更长。VHN、L 和ΔZ：SnF 和 AmF 的作用高于 NaF 和 DIW。L 和ΔZ 随时间没有显著差异（所有处理）。在每个成熟度内，氟化物化合物的 L 和ΔZ 值均显著低于 DIW。