College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Key Laboratory of Marine Environmental and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Bioresour Technol. 2020 Oct;314:123721. doi: 10.1016/j.biortech.2020.123721. Epub 2020 Jun 21.
With depletion of fossil fuel, microalgae is considered as a promising substitute due to high growth rate, efficient cost and high biofuels content. This study investigated the effect of temperature on mixotrophic cultivation of Chlorella vulgaris. In addition, the combination carbon source of inorganic (HCO or CO) and organic (glucose or acetate) for microalgae cultivation was evaluated to obtain the optimum carbon source for mixotrophic cultivation. The results showed that the optimum temperature of microalgae cultivation was at the range of 15-20 °C. The activity of Rubisco was obviously inhibited at the temperature of 30 °C, however, citrate synthase was not susceptible to the increasing temperature. COD removal efficiency was all higher than 64.0%. Low temperature was benefit for protein formation, and the lipid accumulation occurred at high temperature. The results provide a fresh perspective between enzyme activity and temperature variation for product accumulation of microalgae.
随着化石燃料的枯竭，由于生长速度快、成本效率高和生物燃料含量高，微藻被认为是一种很有前途的替代品。本研究探讨了温度对普通小球藻混合营养培养的影响。此外，还评估了无机（HCO 或 CO）和有机（葡萄糖或醋酸盐）组合碳源用于微藻培养，以获得混合营养培养的最佳碳源。结果表明，微藻培养的最佳温度范围在 15-20°C。30°C 时 Rubisco 活性明显受到抑制，而柠檬酸合酶不易受温度升高的影响。COD 去除效率均高于 64.0%。低温有利于蛋白质的形成，而高温则有利于脂质的积累。该结果为微藻产物积累的酶活性与温度变化之间提供了一个新的视角。
