Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
Bioresour Technol. 2022 May;351:127021. doi: 10.1016/j.biortech.2022.127021. Epub 2022 Mar 16.
Microalgal biomass, known as the third generation feedstock for biofuels production, is currently being explored mainly for lipids and functional components. However, the potential of microalgal carbohydrates has not been evaluated. In this investigation, Chlorella vulgaris JSC-6 was used for carbohydrates production from CO and fatty acids under different cultivation strategies to meet the requirements of a CO-neutral and clean fermentation system for biofuel production. Autotrophic cultivation resulted in better carbon assimilation and carbohydrate accumulation; about 1.4 g CO could be converted to 1 g biomass, of which 50% are carbohydrates. Assimilation of fatty acids in photoheterotrophic and mixotrophic modes was influenced by pH, and pH 7-7.5 supported butyrate and acetate assimilation. The maximum carbohydrate content (49.86%) was attained in mixotrophic mode, and the ratio of the simple sugars glucose-xylose-arabinose was 1:0.11:0.02. The higher glucose content makes the microalgal biomass a suitable feedstock for sugar-based fermentations.
微藻生物质被称为生物燃料生产的第三代原料，目前主要针对脂质和功能性成分进行研究。然而，微藻碳水化合物的潜力尚未得到评估。在这项研究中，使用普通小球藻 JSC-6 通过不同的培养策略来生产 CO 和脂肪酸中的碳水化合物，以满足 CO 中性和清洁发酵系统生产生物燃料的要求。自养培养有利于更好地进行碳同化和碳水化合物积累；约 1.4 g CO 可转化为 1 g 生物质，其中 50%为碳水化合物。在异养和混合营养培养方式下，脂肪酸的同化受到 pH 值的影响，pH 值在 7-7.5 时有利于丁酸盐和乙酸盐的同化。在混合营养培养方式下可获得最高的碳水化合物含量（49.86%），单糖葡萄糖-木糖-阿拉伯糖的比例为 1:0.11:0.02。较高的葡萄糖含量使微藻生物质成为适合糖基发酵的原料。
