Department of Chemical & Biological Engineering, Montana State University, Bozeman, MT 59717, United States.
School of Medicine, Stanford University, Stanford, CA 94305, United States.
Bioresour Technol. 2020 Oct;314:123750. doi: 10.1016/j.biortech.2020.123750. Epub 2020 Jun 28.
Prior work has identified that lignins recovered from dilute acid-pretreated corn stover exhibit superior performance in phenol-formaldehyde resins used in wood adhesive applications when compared to diverse process-modified lignins derived from other sources. This improved performance is hypothesized to be due to the higher content of unsubstituted phenolic groups specifically p-coumarate lignin esters. In this work, a diverse set of corn stover samples are employed that exhibit diversity in p-coumarate content and total lignin content to explore the relationship between dilute acid pretreatment conditions, p-coumarate ester hydrolysis, xylan solubilization, and the resulting glucose enzymatic hydrolysis yields. The goal of this study is to identify pretreatment conditions that preserve a significant fraction of the p-coumarate esters while simultaneously achieving high enzymatic hydrolysis yields. Kinetic parameters for p-coumarate ester hydrolysis were quantified and pretreatment-biomass combinations were identified that result in glucose hydrolysis yields of more than 90% while retaining nearly 50 mg p-coumarate/g lignin.
先前的工作已经表明，与其他来源的各种经过工艺改性的木质素相比，从稀酸预处理的玉米秸秆中回收的木质素在用于木材胶黏剂应用的酚醛树脂中表现出更好的性能。这种性能的提高据推测是由于未取代的酚基团，特别是 p-香豆酸木质素酯的含量更高。在这项工作中，使用了一组不同的玉米秸秆样品，这些样品在 p-香豆酸含量和总木质素含量方面表现出多样性，以探讨稀酸预处理条件、p-香豆酸酯水解、木聚糖溶解以及由此产生的葡萄糖酶解产率之间的关系。本研究的目的是确定在同时实现高酶解产率的情况下，能够保留大量 p-香豆酸酯的预处理条件。对 p-香豆酸酯水解的动力学参数进行了定量，并确定了预处理-生物质组合，使葡萄糖水解产率超过 90%，同时保留近 50mg p-香豆酸/g 木质素。
