Department of Chemical Engineering & Materials Science, Michigan State University, East Lansing, MI, USA; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI, USA.
Department of Chemical Engineering & Materials Science, Michigan State University, East Lansing, MI, USA.
Bioresour Technol. 2017 Dec;245(Pt A):242-249. doi: 10.1016/j.biortech.2017.08.200. Epub 2017 Sep 1.
This work investigated the impact of two alkaline pretreatments, ammonia fiber expansion (AFEX) and alkaline hydrogen peroxide (AHP) delignification performed over a range of conditions on the properties of corn stover and switchgrass. Changes in feedstock properties resulting from pretreatment were subsequently compared to enzymatic hydrolysis yields to examine the relationship between enzymatic hydrolysis and cell wall properties. The pretreatments function to increase enzymatic hydrolysis yields through different mechanisms; AFEX pretreatment through lignin relocalization and some xylan solubilization and AHP primarily through lignin solubilization. An important outcome of this work demonstrated that while changes in lignin content in AHP-delignified biomass could be clearly correlated to improved response to hydrolysis, compositional changes alone in AFEX-pretreated biomass could not explain differences in hydrolysis yields. We determined the water retention value, which characterizes the association of water with the cell wall of the pretreated biomass, can be used to predict hydrolysis yields for all pretreated biomass within this study.
本研究考察了两种碱性预处理方法（氨纤维膨胀法（AFEX）和碱性过氧化物法（AHP））在不同条件下对玉米秸秆和柳枝稷性质的影响。预处理后原料性质的变化随后与酶水解产率进行了比较，以考察酶水解与细胞壁性质之间的关系。预处理通过不同的机制来提高酶水解产率；AFEX 预处理通过木质素重定位和一些木聚糖的溶解，而 AHP 主要通过木质素的溶解。这项工作的一个重要结果表明，尽管 AHP 脱木质素生物质中木质素含量的变化可以与水解反应的改善明显相关，但 AFEX 预处理生物质中成分的变化并不能解释水解产率的差异。我们确定了水保留值，它可以用来描述预处理生物质细胞壁与水的结合情况，该值可以用来预测本研究中所有预处理生物质的水解产率。
