Van den Broek M F, Van de Langerijt L G, Van Bruggen M C, Billingham M E, Van den Berg W B
Department of Rheumatology, University Hospital Nijmegen, The Netherlands.
Eur J Immunol. 1992 Jan;22(1):57-61. doi: 10.1002/eji.1830220110.
To investigate the role of CD4+ cells in the induction and maintenance of streptococcal cell wall (SCW)-induced arthritis, Lewis rats were treated with a monoclonal antibody against rat CD4 (W3/25). Injection before onset of the arthritis resulted in resistance to SCW arthritis. Treatment with anti-CD4 during ongoing arthritis induced an amelioration of the arthritis, demonstrating that CD4+ cells are involved in both the induction and effector phases of the chronic arthritis. After return of CD4+ cells to normal levels in the circulation, no arthritis occurred in protected rats, despite the continued presence of SCW in the body. Even reinjection of SCW could not induce arthritis in these rats, suggesting that tolerance to SCW had occurred. In addition, these tolerized rats were refractory to actively induced adjuvant arthritis (AA), but were susceptible to passively transferred AA. Our data imply, that (a) treatment with anti-CD4 plus SCW induces a long-term resistance to SCW-induced arthritis and adjuvant arthritis, (b) SCW and M. tuberculosis may use similar mechanisms of regulation of arthritis and (c) active peripheral suppression is not the mechanism of this nonresponsiveness.
为研究CD4+细胞在链球菌细胞壁（SCW）诱导的关节炎的诱发及维持过程中的作用，给Lewis大鼠注射抗大鼠CD4单克隆抗体（W3/25）。在关节炎发作前注射可使大鼠对SCW关节炎产生抗性。在关节炎持续期间用抗CD4进行治疗可使关节炎病情改善，这表明CD4+细胞参与了慢性关节炎的诱发及效应阶段。在循环中CD4+细胞恢复至正常水平后，尽管体内仍持续存在SCW，但受保护的大鼠未发生关节炎。即使再次给这些大鼠注射SCW也不能诱发关节炎，提示对SCW已产生耐受。此外，这些耐受大鼠对主动诱导的佐剂性关节炎（AA）无反应，但对被动转移的AA敏感。我们的数据表明，（a）用抗CD4加SCW进行治疗可诱导对SCW诱导的关节炎和佐剂性关节炎产生长期抗性，（b）SCW和结核分枝杆菌可能利用相似的关节炎调节机制，以及（c）主动外周抑制不是这种无反应性的机制。
