Monroe J G, Haldar S, Prystowsky M B, Lammie P
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Clin Immunol Immunopathol. 1988 Nov;49(2):292-8. doi: 10.1016/0090-1229(88)90119-5.
While recent evidence from several laboratories has shown that interleukin-4 (IL-4) can act on a number of cells in addition to B lymphocytes, these have thus far been limited to cells of the hematopoietic lineage. Here we report that murine IL-4 promotes DNA synthesis in both primary and immortalized fibroblasts. Marked stimulation of [3H]thymidine incorporation was observed for primary skin fibroblasts or Balb/c3T3 cells stimulated with HPLC- or immunoaffinity-purified as well as recombinant IL-4. Responses to immunoaffinity and recombinant IL-4 were completely blocked with anti-IL-4 antibody. Similar dose/response relationships were observed for recombinant IL-4 on skin fibroblasts and an IL-4 responsive murine T cell tumor, suggesting that the receptors for this lymphokine on these cells is similar. Together, these results show that IL-4 can cause DNA synthesis by murine fibroblasts presumably through ligand-receptor interactions at the cell surface. Implications of these findings to inflammation during an immune response is discussed.
尽管最近几个实验室的证据表明，白细胞介素-4（IL-4）除了作用于B淋巴细胞外，还能作用于许多其他细胞，但迄今为止，这些细胞仅限于造血谱系细胞。在此我们报告，小鼠IL-4能促进原代和成永生化成纤维细胞中的DNA合成。在用HPLC或免疫亲和纯化以及重组IL-4刺激的原代皮肤成纤维细胞或Balb/c 3T3细胞中，观察到显著刺激的[3H]胸腺嘧啶核苷掺入。用抗IL-4抗体可完全阻断对免疫亲和性和重组IL-4的反应。在皮肤成纤维细胞和IL-4反应性小鼠T细胞肿瘤上，重组IL-4表现出相似的剂量/反应关系，这表明这些细胞上这种淋巴因子的受体相似。总之，这些结果表明IL-4可能通过细胞表面的配体-受体相互作用导致小鼠成纤维细胞的DNA合成。本文还讨论了这些发现对免疫反应过程中炎症的影响。
