Dukovich M, Severin J M, White S J, Yamazaki S, Mizel S B
Clin Immunol Immunopathol. 1986 Mar;38(3):381-9. doi: 10.1016/0090-1229(86)90248-5.
Recombinant murine interleukin 1 (IL-1) obtained from a clone of Escherichia coli containing an IL-1 expression plasmid was purified to homogeneity using a sequential extraction procedure and gel filtration chromatography. The purified recombinant IL-1 exhibited a pI of approximately 5.2 and a sp act of 6 X 10(6) units/mg. These values are in agreement with those obtained with natural murine IL-1. The purified recombinant IL-1 enhanced the proliferation of human HEL and WI-38 fibroblasts in a serum-free medium. In addition, IL-1 stimulated fibroblast PGE2 5- to 30-fold over a 24-hr period. The effects of IL-1 on fibroblast activation were obtained with the same concentrations of IL-1 that are effective in the mouse thymocyte assay. These results unequivocally establish the ability of IL-1 to modulate fibroblast proliferation and function.
从含有白细胞介素-1（IL-1）表达质粒的大肠杆菌克隆中获得的重组鼠白细胞介素-1，通过连续提取程序和凝胶过滤色谱法纯化至同质。纯化后的重组IL-1的等电点约为5.2，比活性为6×10⁶单位/毫克。这些值与天然鼠IL-1的值一致。纯化后的重组IL-1在无血清培养基中增强了人HEL和WI-38成纤维细胞的增殖。此外，IL-1在24小时内刺激成纤维细胞产生的前列腺素E2增加了5至30倍。在小鼠胸腺细胞试验中有效的相同浓度的IL-1，可产生对成纤维细胞激活的作用。这些结果明确证实了IL-1调节成纤维细胞增殖和功能的能力。
