• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Novel approach for the prediction of cell densities and viability in standardized translucent cell culture biochips with near infrared spectroscopy.利用近红外光谱预测标准化半透明细胞培养生物芯片中细胞密度和活力的新方法。
Eng Life Sci. 2017 Jan 12;17(5):585-593. doi: 10.1002/elsc.201600162. eCollection 2017 May.
2
Investigation of ifosfamide nephrotoxicity induced in a liver-kidney co-culture biochip.在肝-肾共培养生物芯片中研究异环磷酰胺诱导的肾毒性。
Biotechnol Bioeng. 2013 Feb;110(2):597-608. doi: 10.1002/bit.24707. Epub 2012 Aug 28.
3
On-line near infrared bioreactor monitoring of cell density and concentrations of glucose and lactate during insect cell cultivation.昆虫细胞培养过程中细胞密度和葡萄糖及乳酸浓度的在线近红外生物反应器监测。
J Biotechnol. 2014 Mar 10;173:106-11. doi: 10.1016/j.jbiotec.2014.01.009. Epub 2014 Jan 19.
4
Real-time process monitoring in a semi-continuous fluid-bed dryer - microwave resonance technology versus near-infrared spectroscopy.在半连续流化床干燥器中进行实时过程监测 - 微波共振技术与近红外光谱法的比较。
Int J Pharm. 2018 Feb 15;537(1-2):193-201. doi: 10.1016/j.ijpharm.2017.12.040. Epub 2017 Dec 27.
5
Trends in the development of microfluidic cell biochips for in vitro hepatotoxicity.用于体外肝毒性研究的微流控细胞生物芯片的发展趋势
Toxicol In Vitro. 2007 Jun;21(4):535-44. doi: 10.1016/j.tiv.2006.11.004. Epub 2006 Nov 18.
6
Application of miniaturized near-infrared spectroscopy for quality control of extemporaneous orodispersible films.微型近红外光谱法在即时口腔分散片的质量控制中的应用。
J Pharm Biomed Anal. 2018 Feb 20;150:191-198. doi: 10.1016/j.jpba.2017.11.068. Epub 2017 Dec 22.
7
In situ near-infrared (NIR) versus high-throughput mid-infrared (MIR) spectroscopy to monitor biopharmaceutical production.用于监测生物制药生产的原位近红外（NIR）与高通量中红外（MIR）光谱法
Appl Spectrosc. 2015 Jun;69(6):760-72. doi: 10.1366/14-07588. Epub 2015 May 1.
8
Chemometrics and in-line near infrared spectroscopic monitoring of a biopharmaceutical Chinese hamster ovary cell culture: prediction of multiple cultivation variables.化学计量学和在线近红外光谱监测生物制药中国仓鼠卵巢细胞培养：对多个培养变量的预测。
Talanta. 2013 Jul 15;111:28-38. doi: 10.1016/j.talanta.2013.03.044. Epub 2013 Mar 26.
9
Rapid quantification of intracellular PHA using infrared spectroscopy: an application in mixed cultures.利用红外光谱快速定量细胞内 PHA：在混合培养中的应用。
J Biotechnol. 2010 Nov;150(3):372-9. doi: 10.1016/j.jbiotec.2010.09.939. Epub 2010 Sep 17.
10
Near-infrared spectroscopy: a tool for monitoring submerged fermentation processes using an immersion optical-fiber probe.近红外光谱法：一种使用浸入式光纤探头监测深层发酵过程的工具。
Appl Spectrosc. 2003 Feb;57(2):132-8. doi: 10.1366/000370203321535024.

引用本文的文献

1
Development of a Hybrid Polymer-Based Microfluidic Platform for Culturing Hepatocytes towards Liver-on-a-Chip Applications.用于培养肝细胞以实现芯片上肝脏应用的基于混合聚合物的微流控平台的开发。
Polymers (Basel). 2021 Sep 23;13(19):3215. doi: 10.3390/polym13193215.
2
3D-printed miniaturized fluidic tools in chemistry and biology.化学与生物学领域的3D打印微型流体工具。
Trends Analyt Chem. 2018 Sep;106:37-52. doi: 10.1016/j.trac.2018.06.013. Epub 2018 Jul 5.

本文引用的文献

1
Validation of three viable-cell counting methods: Manual, semi-automated, and automated.三种活细胞计数方法的验证：手动、半自动和自动方法。
Biotechnol Rep (Amst). 2015 Apr 18;7:9-16. doi: 10.1016/j.btre.2015.04.004. eCollection 2015 Sep.
2
Monocyte-induced recovery of inflammation-associated hepatocellular dysfunction in a biochip-based human liver model.基于生物芯片的人肝模型中单核细胞诱导的炎症相关肝细胞功能障碍的恢复
Sci Rep. 2016 Feb 23;6:21868. doi: 10.1038/srep21868.
3
A new fluorescent dye for cell tracing and mitochondrial imaging in vitro and in vivo.一种用于体外和体内细胞追踪及线粒体成像的新型荧光染料。
J Biophotonics. 2016 Sep;9(9):888-900. doi: 10.1002/jbio.201500190. Epub 2015 Nov 13.
4
A microfluidically perfused three dimensional human liver model.一种微流控灌注的三维人肝脏模型。
Biomaterials. 2015 Dec;71:119-131. doi: 10.1016/j.biomaterials.2015.08.043. Epub 2015 Aug 25.
5
Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions.用于培养具有改善灌注条件的内皮细胞层的微流控支持生物芯片设计。
Biofabrication. 2015 Mar 2;7(1):015013. doi: 10.1088/1758-5090/7/1/015013.
6
Measurement and control of pressure driven flows in microfluidic devices using an optofluidic flow sensor.使用光流体流量传感器对微流控设备中压力驱动流进行测量与控制。
Biomicrofluidics. 2014 Oct 24;8(5):054123. doi: 10.1063/1.4900523. eCollection 2014 Sep.
7
In situ near infrared spectroscopy monitoring of cyprosin production by recombinant Saccharomyces cerevisiae strains.重组酿酒酵母菌株产环孢菌素的原位近红外光谱监测
J Biotechnol. 2014 Oct 20;188:148-57. doi: 10.1016/j.jbiotec.2014.07.454. Epub 2014 Aug 10.
8
A novel ultrathin collagen nanolayer assembly for 3-D microtissue engineering: Layer-by-layer collagen deposition for long-term stable microfluidic hepatocyte culture.一种用于三维微组织工程的新型超薄胶原纳米层组件：逐层胶原沉积用于长期稳定的微流控肝细胞培养。
Technology (Singap World Sci). 2014 Mar;2(1):67-74. doi: 10.1142/S2339547814500083.
9
Automated and online characterization of adherent cell culture growth in a microfabricated bioreactor.微制造生物反应器中贴壁细胞培养生长的自动化在线表征
J Lab Autom. 2014 Oct;19(5):437-43. doi: 10.1177/2211068214529288. Epub 2014 Apr 1.
10
A bioartificial renal tubule device embedding human renal stem/progenitor cells.一种嵌入人肾干细胞/祖细胞的生物人工肾小管装置。
PLoS One. 2014 Jan 30;9(1):e87496. doi: 10.1371/journal.pone.0087496. eCollection 2014.

利用近红外光谱预测标准化半透明细胞培养生物芯片中细胞密度和活力的新方法。

Novel approach for the prediction of cell densities and viability in standardized translucent cell culture biochips with near infrared spectroscopy.

作者信息

Gröger Marko, Lange Matthias, Rennert Knut, Kaschowitz Tobias, Plettenberg Holger, Hoffmann Martin, Mosig Alexander S

机构信息

Center for Sepsis Control and Care Jena University Hospital Jena Germany.

Institute of Biochemistry II Jena University Hospital Jena Germany.

出版信息

Eng Life Sci. 2017 Jan 12;17(5):585-593. doi: 10.1002/elsc.201600162. eCollection 2017 May.

DOI:10.1002/elsc.201600162
PMID:32624804
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6999322/
Abstract

Near infrared spectroscopy is a rapid and nondestructive method for compositional analysis of biological material. The technology is widely used within bioreactors and possesses potential as a standardized method for quality control in miniaturized microfluidic cell culture systems. Here, we established a method for quantification of cell density and viability of adherent HepaRG cells cultured in a translucent, miniaturized cell culture biochip. The newly developed statistical models for interpretation of near infrared spectroscopy from biochips are the basis for a novel method of fast, continuous, and contact-free analysis of cell viability and real-time monitoring of cell growth. The technique thus paves the way for a robust and reliable high-throughput analysis of biochip-embedded cell cultures.

摘要

近红外光谱法是一种用于生物材料成分分析的快速无损方法。该技术在生物反应器中被广泛应用，并且具有作为小型微流控细胞培养系统质量控制标准化方法的潜力。在此，我们建立了一种用于定量培养在半透明小型化细胞培养生物芯片中的贴壁HepaRG细胞的细胞密度和活力的方法。新开发的用于解释来自生物芯片的近红外光谱的统计模型是一种快速、连续且非接触式分析细胞活力和实时监测细胞生长的新方法的基础。因此，该技术为对生物芯片中嵌入的细胞培养物进行稳健可靠的高通量分析铺平了道路。