Irdahayu Nik Murniati Nik Man, Shantini Kannusamy, Huong Kai-Hee, Vigneswari Sevakumaran, Aziz Nursolehah Abdul, Azizan Mohd Noor Mohd, Amirul Al-Ashraf Abdullah
Malaysian Institute of Pharmaceuticals & Nutraceuticals Halaman Bukit Gambir Penang Malaysia.
School of Biological Sciences Universiti Sains Malaysia Minden Penang Malaysia.
Eng Life Sci. 2017 Jun 27;17(9):1050-1059. doi: 10.1002/elsc.201600217. eCollection 2017 Sep.
Separation of poly(3-hydroxybutyrate--4-hydroxybutyrate) [P(3HB--4HB)] from bacterial cell matter is a critical step in the downstream process with respect to material quality and eco-balance as P(3HB--4HB) is widely used for biomedical applications. Therefore, an efficient and eco-based extraction of P(3HB--4HB) using a combination of NaOH and Lysol in digesting the non-polymeric cell material (NPCM) digestion is developed. The NaOH and Lysol show synergistic influence on the copolymer extraction at a high purity and recovery of 97 and 98 wt% respectively. The optimized cell digestion method was found applicable to a vast batch of cells containing copolymers from various 4HB monomer compositions. At the largest extraction volume of 100 L, P(3HB--4HB) with a purity of 89 wt% was extracted with a maximum recovery of 90 wt%. The method developed has also eliminated the cell pretreatment step. The extraction method developed in this research has not only produced an economic and efficient copolymer recovery but has also retained the copolymer quality, in term of its molecular weight and thermal properties. It demonstrates a practical and promising downstream processing method in recovering the copolymer effectively from the bacterial biomass.
从细菌细胞物质中分离聚（3-羟基丁酸酯-4-羟基丁酸酯）[P(3HB-4HB)]是下游加工过程中的关键步骤，这对于材料质量和生态平衡至关重要，因为P(3HB-4HB)被广泛用于生物医学应用。因此，开发了一种在消化非聚合细胞物质（NPCM）时使用NaOH和来苏尔组合的高效且基于生态的P(3HB-4HB)提取方法。NaOH和来苏尔对共聚物提取具有协同影响，纯度和回收率分别高达97 wt%和98 wt%。发现优化后的细胞消化方法适用于大量含有来自各种4HB单体组成的共聚物的细胞。在最大提取体积为100 L时，提取出了纯度为89 wt%的P(3HB-4HB)，最大回收率为90 wt%。所开发的方法还省去了细胞预处理步骤。本研究中开发的提取方法不仅实现了经济高效的共聚物回收，还在分子量和热性能方面保留了共聚物的质量。它展示了一种从细菌生物质中有效回收共聚物的实用且有前景的下游加工方法。
