固定化脂肪酶在酯化反应中的工程学方面：特别强调拥挤、限制和扩散效应。
Engineering aspects of immobilized lipases on esterification: A special emphasis of crowding, confinement and diffusion effects.
作者信息
Parashar Surendra Kumar, Srivastava Sunil Kumar, Dutta N N, Garlapati Vijay Kumar
机构信息
Department of Chemical Engineering/Chemistry Jaypee University of Engineering and Technology Guna Madhya Pradesh India.
Department of Biotechnology and Bioinformatics Jaypee University of Information Technology Waknaghat Himachal Pradesh India.
出版信息
Eng Life Sci. 2018 Feb 16;18(5):308-316. doi: 10.1002/elsc.201700082. eCollection 2018 May.
Cross-linked enzyme crystal (CLEC) and sol-gel entrapped pseudomonas sp. lipase were investigated for the esterification of lauric acid with ethanol by considering the effects of reaction conditions on reaction rate. The activation energy for the reaction was estimated to be 1097.58 J/mol and 181.75 J/mol for sol-gel and CLEC entrapped lipase respectively. CLEC lipase exhibited a marginal internal diffusion effect on reaction rate over sol-gel lipases and found to be interesting. The overall reaction mechanism was found to conform to the Ping Pong Bi Bi mechanism. The higher efficiency of sol-gel lipases over CLEC lipases in esterification reaction is mainly due to the combined effects of crowding, confinement and diffusional limitations.
通过考虑反应条件对反应速率的影响，研究了交联酶晶体（CLEC）和溶胶-凝胶包埋的假单胞菌属脂肪酶催化月桂酸与乙醇的酯化反应。溶胶-凝胶包埋脂肪酶和CLEC包埋脂肪酶催化该反应的活化能分别估计为1097.58焦耳/摩尔和181.75焦耳/摩尔。与溶胶-凝胶脂肪酶相比，CLEC脂肪酶对反应速率的内部扩散效应较小，这一现象值得关注。发现整个反应机制符合乒乓双双机制。溶胶-凝胶脂肪酶在酯化反应中比CLEC脂肪酶具有更高的效率，这主要是由于拥挤、受限和扩散限制的综合作用。
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