Campbell A M, Lowe P A
Biochem J. 1977 Apr 1;163(1):177-9. doi: 10.1042/bj1630177.
The sigma-factor of Escherichia coli RNA polymerase was shown to dissociate from the core enzyme as a function of absolute concentration. The association constant is in the range 10(6)-10(8) litre/mol. This implies that the amount of holoenzyme, core enzyme and sigma-factor in RNA polymerase assays may vary according to the absolute concentration of the enzyme.
已表明大肠杆菌RNA聚合酶的σ因子会作为绝对浓度的函数与核心酶解离。缔合常数在10⁶ - 10⁸升/摩尔范围内。这意味着在RNA聚合酶测定中全酶、核心酶和σ因子的量可能会根据酶的绝对浓度而变化。
