Campbell A M
Biochem J. 1976 Apr 1;155(1):101-5. doi: 10.1042/bj1550101.
Laser light-scattering studies of bacteriophage PM2 DNA showed the molecule to have mol.wt. 5.9 X 10(6) and root-mean -square radius 125 nm at an ionic strength of 0.2 mol/litre. Computer-generated curves compatible with these data were compared with the experimental interference curve for several structural models of the molecules. The data fit best to an asymmetric four-armed planar molecule in which all four arms emerge from or close to the one area of the molecule. This contrasts with the smaller DNA molecules investigated, which have shown a three-armed molecule, whose symmetry varies with primary structure.
对噬菌体PM2 DNA的激光光散射研究表明,在离子强度为0.2摩尔/升时,该分子的分子量为5.9×10⁶,均方根半径为125纳米。将与这些数据相符的计算机生成曲线与该分子几种结构模型的实验干涉曲线进行了比较。数据最符合一种不对称的四臂平面分子,其中所有四条臂都从分子的一个区域或靠近该区域伸出。这与所研究的较小DNA分子形成对比,较小的DNA分子呈现出一种三臂分子,其对称性随一级结构而变化。