PM2噬菌体脱氧核糖核酸的构象分析。大小对超螺旋形状的影响。
Conformational analysis of deoxyribonucleic acid from PM2 bacteriophage. The effect of size on supercoil shape.
作者信息
Campbell A M
出版信息
Biochem J. 1976 Apr 1;155(1):101-5. doi: 10.1042/bj1550101.
Abstract
Laser light-scattering studies of bacteriophage PM2 DNA showed the molecule to have mol.wt. 5.9 X 10(6) and root-mean -square radius 125 nm at an ionic strength of 0.2 mol/litre. Computer-generated curves compatible with these data were compared with the experimental interference curve for several structural models of the molecules. The data fit best to an asymmetric four-armed planar molecule in which all four arms emerge from or close to the one area of the molecule. This contrasts with the smaller DNA molecules investigated, which have shown a three-armed molecule, whose symmetry varies with primary structure.
摘要
对噬菌体PM2 DNA的激光光散射研究表明，在离子强度为0.2摩尔/升时，该分子的分子量为5.9×10⁶，均方根半径为125纳米。将与这些数据相符的计算机生成曲线与该分子几种结构模型的实验干涉曲线进行了比较。数据最符合一种不对称的四臂平面分子，其中所有四条臂都从分子的一个区域或靠近该区域伸出。这与所研究的较小DNA分子形成对比，较小的DNA分子呈现出一种三臂分子，其对称性随一级结构而变化。
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本文引用的文献
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Locating interrupted hydrogen bonding in the secondary structure of PM2 circular DNA by comparative denaturation mapping.通过比较变性图谱定位PM2环状DNA二级结构中的间断氢键。
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