Theron A J, Anderson R
Department of Medical Microbiology, University of Pretoria, Republic of South Africa.
Int J Vitam Nutr Res. 1988;58(2):218-24.
The effects of oral administration of ascorbate (3 grams daily for 14 days) on the elastase inhibitory capacity (EIC) of serum alpha-1-protease inhibitor (API) and on the profile of leukoattractant (FMLP) activated luminol-enhanced chemiluminescence (LECL) responses of blood from 29 cigarette smokers were investigated in a placebo-controlled, doubleblind crossover trial. Relative to non-smokers the EIC's of serum API from cigarette smokers showed no detectable functional inactivation. However the blood LECL responses were significantly greater in smokers than in non-smokers (p less than 0.005). Serum EIC activity remained unchanged during the administration of ascorbate to cigarette smokers. However the early-occurring extracellular LECL responses of FMLP-activated blood from cigarette smokers declined significantly during intake of ascorbate by the 29 cigarette smokers. These inhibitory effects of ascorbate on the extracellular LECL responses were highly significant (p less than 0.005) in smokers (15/29) with elevated (1 X SD higher than the mean value for non-smokers) extracellular LECL responses, whilst the corresponding values in smokers with normal LECL responses remained unchanged during ascorbate administration. These results show that measurement of serum EIC capacity in the setting of normal API levels is of no value in the detection of smokers at high-risk for the development of smoking-related diseases or in the measurement of the potential protective activity of anti-oxidant supplementation. However, measurement of FMLP-activated LECL responses of whole blood may be useful in achieving these objectives.
在一项安慰剂对照、双盲交叉试验中，研究了口服抗坏血酸盐（每日3克，共14天）对29名吸烟者血清α-1-蛋白酶抑制剂（API）的弹性蛋白酶抑制能力（EIC）以及对白三烯趋化因子（FMLP）激活的鲁米诺增强化学发光（LECL）血液反应谱的影响。相对于不吸烟者，吸烟者血清API的EIC未显示出可检测到的功能失活。然而，吸烟者的血液LECL反应明显高于不吸烟者（p<0.005）。在向吸烟者施用抗坏血酸盐期间，血清EIC活性保持不变。然而，在29名吸烟者摄入抗坏血酸盐期间，FMLP激活的吸烟者血液的早期细胞外LECL反应显著下降。抗坏血酸盐对细胞外LECL反应的这些抑制作用在细胞外LECL反应升高（比不吸烟者的平均值高1个标准差）的吸烟者（15/29）中非常显著（p<0.005），而在LECL反应正常的吸烟者中，相应值在施用抗坏血酸盐期间保持不变。这些结果表明，在API水平正常的情况下测量血清EIC能力对于检测有吸烟相关疾病高风险的吸烟者或测量抗氧化剂补充的潜在保护活性没有价值。然而，测量全血的FMLP激活的LECL反应可能有助于实现这些目标。
