Liu Xu-Sheng, Yuan Ling-Ling, Gao Yan, Zhou Lu-Meng, Yang Jian-Wei, Pei Zhi-Jun
Department of Nuclear Medicine and Institute of Anesthesiology and Pain, Taihe Hospital, Hubei University of Medicine, Shiyan, 44200, China.
Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, China.
J Cancer. 2020 Jun 7;11(16):4851-4860. doi: 10.7150/jca.44754. eCollection 2020.
To investigate the expression of methyltransferase 3 (METTL3) and its relationship with F-FDG uptake in patients with esophageal carcinoma (ESCA). This study analyzed the expression of METTL3 in ESCA and its relationship with clinicopathological features by The Cancer Genome Atlas (TCGA) database. Immunohistochemical staining was performed on 57 tumor tissues of ESCA patients who underwent PET/CT scan before surgery to evaluate the expression of METTL3, glucose transporter 1 (GLUT1), and hexokinase 2 (HK2) in tumor tissues and peritumoral tissues. Analyze the relationship between SUVmax with METTL3, HK2, and GLUT1 expression. The expression of METTL3, GLUT1, and HK2 was significantly increased in ESCA tissues compared with normal tissues ( 0.001). The expression of METTL3 was correlated with tumor size and histological differentiation ( 0.05), and there was no significant difference between age, sex, pathological types, tumor staging, or lymph node metastasis ( > 0.05). The SUVmax was significantly higher in tumors with high METTL3 expression (17.822±6.249) compared to low METTL3 expression (9.573±5.082) ( 0.001). There was a positive correlation between the SUVmax and METTL3 expression in ESCA ( = 0.647, 0.001). Multivariate analysis confirmed the association between SUVmax and METTL3 expression ( 0.05). GLUT1 and HK2 expression in ESCA was positively correlated with F-FDG uptake and METTL3 status ( 0.001). The high expression of METTL3 is related to the high SUVmax in ESCA, and METTL3 may increase F-FDG uptake by regulating GLUT1 and HK2.
探讨甲基转移酶3（METTL3）在食管癌（ESCA）患者中的表达及其与F-FDG摄取的关系。本研究通过癌症基因组图谱（TCGA）数据库分析了METTL3在ESCA中的表达及其与临床病理特征的关系。对57例术前接受PET/CT扫描的ESCA患者的肿瘤组织进行免疫组织化学染色，以评估肿瘤组织和瘤周组织中METTL3、葡萄糖转运蛋白1（GLUT1）和己糖激酶2（HK2）的表达。分析最大标准摄取值（SUVmax）与METTL3、HK2和GLUT1表达之间的关系。与正常组织相比，ESCA组织中METTL3、GLUT1和HK2的表达显著增加（P<0.001）。METTL3的表达与肿瘤大小和组织学分化相关（P<0.05），而在年龄、性别、病理类型、肿瘤分期或淋巴结转移方面无显著差异（P>0.05）。与低METTL3表达（9.573±5.082）的肿瘤相比，高METTL3表达的肿瘤中SUVmax显著更高（17.822±6.249）（P<0.001）。ESCA中SUVmax与METTL3表达呈正相关（r = 0.647，P<0.001）。多因素分析证实了SUVmax与METTL3表达之间的关联（P<0.05）。ESCA中GLUT1和HK2的表达与F-FDG摄取和METTL3状态呈正相关（P<0.001）。METTL3的高表达与ESCA中高SUVmax相关，并且METTL3可能通过调节GLUT1和HK2增加F-FDG摄取。
