Zhang Y, Ramos B F, Jakschik B, Baganoff M P, Deppeler C L, Meyer D M, Widomski D L, Fretland D J, Bolanowski M A
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110.
Inflammation. 1995 Feb;19(1):119-32. doi: 10.1007/BF01534385.
In the reverse passive Arthus reaction in mouse skin and immune injury of mouse dermal basement membrane, neutrophil (PMN) infiltration in mast cell deficient WBB6F1-W/Wv (W/Wv) mice was only 40% of that in WBB6F1-(+)/+ (+/+) mice that had a normal mast cell repertoire. An anti-tumor necrosis factor-alpha (TNF-alpha) monoclonal antibody (mAb) decreased PMN infiltration by 35-80% in +/+ but not W/Wv mice. In addition, an anti-human interleukin-8 (IL-8) MAb, DM/C7, inhibited PMN infiltration of the skin induced by either intradermal administration of recombinant human IL-1 beta or immune complex deposition. In both models of immune complex injury, DM/C7 reduced PMN infiltration by 40-60% in +/+ mice but not W/Wv mice. PMN infiltration and the sensitivity of this infiltration to anti-TNF-alpha or DM/C7 MAb in W/Wv mice whose mast cell population had been restored was indistinguishable from the influx observed in +/+ mice. These data suggest that TNF-alpha, IL-8, and mast cells play a fundamental role in PMN recruitment following immune complex injury.
在小鼠皮肤的被动反向阿瑟斯反应以及小鼠真皮基底膜的免疫损伤中，在肥大细胞缺陷的WBB6F1-W/Wv（W/Wv）小鼠中，中性粒细胞（PMN）浸润仅为具有正常肥大细胞库的WBB6F1-(+)/+（+/+）小鼠的40%。一种抗肿瘤坏死因子-α（TNF-α）单克隆抗体（mAb）使+/+小鼠的PMN浸润减少35%-80%，但对W/Wv小鼠无效。此外，一种抗人白细胞介素-8（IL-8）单克隆抗体DM/C7，可抑制由皮内注射重组人IL-1β或免疫复合物沉积所诱导的皮肤PMN浸润。在两种免疫复合物损伤模型中，DM/C7使+/+小鼠的PMN浸润减少40%-60%，但对W/Wv小鼠无效。在肥大细胞群体已恢复的W/Wv小鼠中，PMN浸润以及这种浸润对抗TNF-α或DM/C7单克隆抗体的敏感性与在+/+小鼠中观察到的流入情况无明显差异。这些数据表明，TNF-α、IL-8和肥大细胞在免疫复合物损伤后的PMN募集过程中起重要作用。
