Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
Mol Med Rep. 2020 Aug;22(2):715-722. doi: 10.3892/mmr.2020.11137. Epub 2020 May 7.
The present study aimed to investigate the effects of human umbilical cord mesenchymal stem cells (Huc‑MSCs)‑derived exosomes on the migratory abilities of endometrial glandular epithelial cells, and to evaluate the underlying mechanism from the perspective of epithelial‑mesenchymal transition (EMT). Huc‑MSCs were prepared from human umbilical cord, and eutopic endometrial glandular epithelial cells were isolated from patients with endometriosis. The exosomes derived from Huc‑MSCs (Huc‑MSCs‑exo) were prepared using an exosome extraction kit. The endometrial glandular epithelial cells were randomly divided into two groups: Huc‑MSCs‑exo and control. Cell migratory ability was assessed and western blotting was used to detect the expression levels of EMT. The results of the present study demonstrated that Huc‑MSCs‑exo treatment significantly enhanced the migration of endometrial glandular epithelial cells from patients with endometriosis (P<0.05). The present study also demonstrated that treatment with Huc‑MSCs‑exo inhibited the expression levels of E‑cadherin and promoted the expression levels of Vimentin and N‑cadherin at both the mRNA and protein level. The results of the current study indicate that Huc‑MSCs‑exo enhance the migratory ability of endometrial glandular epithelial cells via promotion of EMT.
本研究旨在探讨人脐带间充质干细胞（Huc-MSCs）衍生的外泌体对子宫内膜腺上皮细胞迁移能力的影响，并从上皮-间充质转化（EMT）的角度评估其潜在机制。从人脐带中分离培养出 Huc-MSCs，从子宫内膜异位症患者中分离出在位子宫内膜腺上皮细胞。使用外泌体提取试剂盒提取 Huc-MSCs 衍生的外泌体（Huc-MSCs-exo）。将子宫内膜腺上皮细胞随机分为两组：Huc-MSCs-exo 和对照组。评估细胞迁移能力，并通过 Western blot 检测 EMT 的表达水平。本研究结果表明，Huc-MSCs-exo 处理显著增强了子宫内膜异位症患者子宫内膜腺上皮细胞的迁移能力（P<0.05）。本研究还表明，Huc-MSCs-exo 处理抑制 E-钙黏蛋白的表达水平，并在 mRNA 和蛋白水平上促进波形蛋白和 N-钙黏蛋白的表达水平。本研究结果表明，Huc-MSCs-exo 通过促进 EMT 增强子宫内膜腺上皮细胞的迁移能力。
