Speiser F, Weiss N
Experientia. 1979 Nov 15;35(11):1512-4. doi: 10.1007/BF01962817.
112 sera from Europeans with parasitologically proven helminthiasis were tested in the enzyme-linked immunosorbent assay (E.L.I.S.A.) against 6 crude extracts of various helminths (2 of adult worms: Dipetalonema viteae, Fasciola hepatica; 3 of eggs: Ascaris suum, Toxocara canis, Schistosoma mansoni; and of Echinococcus granulosus scolices) and against bovine hydatid fluid. Each serum was tested simultaneously at a fixed dilution of 1:160 against all antigens. Extensive cross-reactions were observed, leading to the conclusion that non-purified helminth antigens, even in combination, are of limited value for reliable serodiagnosis in E.L.I.S.A.
对112份经寄生虫学证实患有蠕虫病的欧洲人的血清进行了酶联免疫吸附测定（ELISA），检测其针对6种不同蠕虫的粗提物（2种成虫提取物：罗阿丝虫、肝片吸虫；3种虫卵提取物：猪蛔虫、犬弓首蛔虫、曼氏血吸虫；以及细粒棘球绦虫头节提取物）和牛包虫液的反应。每份血清均以1:160的固定稀释度同时针对所有抗原进行检测。观察到广泛的交叉反应，得出结论：即使是组合使用，未纯化的蠕虫抗原在ELISA中用于可靠的血清诊断价值有限。
